胎盘组织TLR3和TLR9表达与HBV宫内感染的关系

The Expression of Toll-like Receptor 3 and 9 in Human Placenta and Their Role in the HBV Intrauterine Infection

目的探讨外周血乙肝表面(HBsAg)阳性对胎盘组织Toll样受体3(TLR3)和Toll样受体9(TLR9)表达的影响。方法收集HBsAg阳性母亲的足月胎盘组织24例和HBsAg阴性母亲的足月胎盘组织6例。采用SABC免疫组织化学方法检测胎盘组织HBsAg、TLR3、TLR9的表达。结果 HBsAg阳性母亲的胎盘组织HBsAg阳性率为75.0%,阳性信号位于蜕膜细胞、滋养层细胞、间质细胞和绒毛毛细血管内皮细胞。HBsAg阳性胎盘有少量的TLR3阳性表达,主要在滋养层细胞和间质细胞,而HBsAg阴性母亲胎盘组织中未检测到TLR3的表达。在HBsAg阳性胎盘的滋养层细胞、间质细胞和绒毛毛细血管内皮细胞都有TLR9的强阳性表达,HBsAg阴性胎盘的滋养层细胞中也检测到TLR9,但阳性信号弱。在同一HBsAg阳性的胎盘组织中,TLR9的表达及分布比TLR3更强、更广泛。结论外周血中HBV可能激活和增强胎盘细胞的TLR9的表达;但对TLR3可能仅有微弱的激活作用或无激活作用。TLR9、TLR3的表达可能与胎盘感染、胎儿宫内感染乙肝病毒(HBV)有一定的关系,但两者之间的因果关系有待进一步研究。

Objective To explore on the expression of toll-like receptor 3（TLR3） and toll-like receptor 9（TLR9） in human placenta and their role in the HBV intrauterine infection. Methods 24 full-term placental tissues of HBsAg carriers and 6 of HBsAg sero-negative pregnant women were collected.The HBsAg,TLR3 and TLR9 in the placental tissues were investigated by Strept Anidin-Biotin peroxidase Complex（SABC） immunohistochemical staining. Results The HBsAg positive rate in the full-time placental tissues from HBsAg carrier pregnant women was 75.00%.The positive signals of HBsAg could locate in the cytoplasms of decidual cells,trophoblastic cells,villous mesenchymocytes and villous capillary endothelial cells.The positive signals of TLR3 mainly locate in the cytoplasm of trophoblastic cells and villous mesenchymocytes in the HBsAg-positive placental tissues.TLR3 could not be found in the placenta of HBsAg sero-negative pregnant women.While the positive signals of TLR9 could locate in the cytoplasm of trophoblastic cells,villous mesenchymocytes and villous capillary endothelial cells in the HBsAg-positive placental.The trophoblastic cells in the placentas of HBsAg sero-negative pregnant women also expressed TLR9.But the positive signals of TLR9 in the HBsAg positive placental were stronger than in the HBsAg-negative placentas.In a HBsAg-positive placenta,the expression and distribution of TLR9 were stronger and more expanding than TLR3. Conclusion HBV stimulation may possibly activate and strongly enhance TLR9 expression in placenta.Whereas there may be only weak or no response of TLR3 to HBV stimulation.The expression of TLR3 and TLR9 in human placenta may play the important role in the HBV intrauterine infection.