羊骨酶解发酵液钙鳌合物对骨质疏松大鼠骨密度和骨代谢生化指标的影响

Effect of SBEF-Ca on Bone Mineral Density and Biochemical Parameters of Bone Metabolism in Rats with Osteoporosis

目的探讨羊骨酶解发酵液钙螯合物(SBEF-Ca)对雌激素缺乏造成的骨质疏松的防治作用。方法 40只3.5月龄的Sprague-Dawley大鼠,按体重随机分为正常组(假手术+蒸馏水)、模型对照组(去卵巢+蒸馏水)和高、中、低3个剂量组(去卵巢+SBEF-Ca)。术后12 d,持续灌胃11周后,测定股骨密度、长度以及血清中反映骨代谢的主要生化指标。结果模型对照组大鼠股骨密度和长度显著减小,各剂量组均可扭转这种减小趋势并使股骨密度和长度维持在正常组水平,其中高剂量组的股骨密度显著大于中、低剂量组;各剂量组的股骨长度差异不显著;所有大鼠的血Ca、血P水平差异无显著性;模型对照组的血清碱性磷酸酶(ALP)、骨钙素(BGP)水平明显升高,而各剂量组的ALP、BGP水平与正常组差异不显著,且高剂量组ALP水平显著低于中、低剂量组,中、高剂量组的BGP水平(P>0.05)显著低于低剂量组。结论低、中、高剂量的SBEF-Ca对绝经后骨质疏松症有预防作用。

Objective The purpose of this study was to investigate the preventive and therapeutic role of sheep bone enzymatic fermentation-calcium chelate（SBEF-Ca） against osteoporosis.Methods Forty 3.5-month-old Sprague-Dawley rats were randomly divided into normal group（sham ＋ water,ig）,model control group（ovariectomized ＋ water,ig）,and three dosage groups（ovariectomized ＋ SBEF-Ca,ig）.From 12 days after ovariectomy,the rats were treated with water or SBEF-Ca intragastically,respectively,for 11 weeks.Then the femoral bone mineral density（BMD） and femoral length,as well as the main parameters of bone metabolism were measured.Results Both the femoral BMD and the length were significantly decreased in the model control group,and all the dosage groups reversed the declining tendency and brought them to the level of normal group.BMD of the high-dosage group was markedly higher than that of model group and the other two dosage groups.There were no statistically significant differences in the femur length among dosage groups.The serum Ca and P levels of all rats had no significant differences.An apparent rise of alkaline phosphatase（ALP） and bone gla protein（BGP） occurred in the model control group,but these two parameters of all dosage groups had no significant differences compared with those of the normal group（P 0.05）.ALP level of the high-dose group was significantly lower than that of the low and medium dosage groups.The BGP levels of the medium and highdosage groups were significantly lower than that of the low dose group.Conclusion All the low,medium and high dosages of SBF-Ca show preventive effect on the occurrence of postmenopausal osteoporosis in rats.