5-氮杂脱氧胞苷和苯丁酸钠对卵巢癌细胞SKOV3的影响

Effects of 5-Aza-2'-deoxycytidine and sodium 4-phenylbutyrate on ovarian cancer cell line SKOV3

目的观察5-氮杂脱氧胞苷(5-Aza-2'-CdR)和苯丁酸钠(SPB)对卵巢癌细胞SKOV3生长及侵袭能力的影响。方法应用相差显微镜观察SKOV3用5-Aza-2'-CdR和SPB处理前后细胞的形态变化;应用细胞生长曲线、四甲基偶氮唑蓝(MTT)比色法观察细胞生长的抑制作用;采用流式细胞术测定细胞周期分布的变化;用Transwell小室法检测药物处理前后细胞侵袭力的变化。结果 5-Aza-2'-CdR和SPB联用时,卵巢癌细胞SKOV3的核形态变化明显,细胞的生长速度明显减慢,细胞周期被阻滞在G0/G1期,S期细胞数减少;药物处理后SKOV3卵巢癌细胞的侵袭能力降低与对照组、单用药组相比,P<0.05。结论 5-Aza-2'-CdR和SPB联用时有明显的增效作用。

[Objective]To study the inducing and invasion ability effects of DNA methyhransferase inhibitor 5Aza-2＇-deoxycytidine （5-Aza-2＇-CdR） and histone deacetylase inhibitor sodium 4-phenylbutyrate （SPB） on ovarian cancer cell line SKOV3. [Methods]Ovarian cancer cell line SKOV3 was treated by 5-Aza-2′-deoxycytidines and sodium 4-phenylbutyrate, the cells morphosis were observed by phase contrast microscope. Cellular proliferation was determined by the cell growth curve and MTT assay. The cell cycle were analyzed by flow cytometry. The invasion ability of SKOV3 cells was tested by transwell chamber assay.[Results]Compared with the control and Single drug group, SKOV3 cell displayed differentiation tendency of morphology, such as decreased atypia and nucleus to cytoplasm ratio,the cell growth speed was significantly slowed down ,the cell cycle was arrested at G0/G1 phase and cell number in S phase was decreased after 5-Aza-2＇-CdR combined with SPB treatment （P 0.05）. The invasion ability of SKOV3 cell decreased after 5-Aza-2＇-CdR combined with SPB treatment （P 0.05）.[Conclusion]The combining DNA methyhransferase inhibitors with histone deacetylase inhibitors have an obvious synergistic effects of induction and invasive ability.