化学发光酶免疫法与高效液相色谱法测定血清茶碱浓度的比较

Comparison of CLEIA and HPLC for Determining Serum Theophylline Concentration

目的探讨化学发光酶免疫(CLEIA)法与高效液相色谱(HPLC)法测定人血清中茶碱质量浓度的相关性。方法分别用CLEIA法和HPLC法平行测定24例老年慢性阻塞性肺疾病患者的茶碱血清质量浓度,比较两种方法的测定结果。结果两者测定值经配对t检验,P>0.05;经回归分析,呈线性关系,回归方程为Y=0.2831+1.069X,r=0.9883(n=24),茶碱质量浓度在1.0～80.0mg/L范围内与峰面积线性关系良好,差异无显著意义(P<0.05)。结论用CLEIA法和HPLC法测定的茶碱血清浓度,具有良好的相关性。

Objective To research the relativity of results of serum theophylline concentration determined by CLEIA and HPLC.Methods Serum theophylline concentrations in 24 cases of senile chronic obstructive pulmonary disease were determined by CLEIA and HPLC separately,and the results were compared.Results Both determination values were tested by paired t-test for dependent sample（P 0.05）.The analysis of correlation and regression showed the linear relation between CLEIA and HPLC methods.The regression equation was Y = 0.283 1 ＋ 1.069 X,r = 0.988 3,（n = 24）.The linear range was 1.0-80.0 mg /L and no significant differences between 2 groups（P 0.05） were found.Conclusion The relativity is good between the serum theophylline concentrations determined by CLEIA and HPLC.