高效液相色谱法测定复方洛美沙星即型凝胶滴耳剂中地塞米松含量

Determination of Dexamethasone in Compound Lomefloxac in Situ Forming Gels Ear Drops by HPLC

目的采用高效液相色谱法测定复方洛美沙星即型凝胶滴耳剂中地塞米松的含量。方法以Diamonsil C18柱为色谱柱,流动相为乙腈-水(40:60,pH为3.0～3.5),流速为1mL/min,柱温40℃,检测波长240nm,进样量20μL。以峰面积外标法计算。结果洛美沙星质量浓度在0.015～0.036mg/mL范围内与峰面积线性关系良好,r=0.9999(n=5),平均回收率为98.01%,RSD=2.95%(n=9)。结论该法操作简便、快速,结果准确可靠,重现性好,可用于测定复方洛美沙星即型凝胶滴耳剂中地塞米松的含量。

Objective To establish a HPLC method for the determination of dexamethasone in Compound Lomefloxacin Ear Drops.Methods The Diamonsil C18 column was used as an analytical column.The mobile phase was acetonitrile-water(40:60) with the flow rate of 1 mL/min,the detective wavelength was 240 nm.The external standard method was used.Results The standard curve of dexamethasone was linear in the concentration range of 0.015-0.036 mg /mL(r = 0.999 9),the average recovery rate was 98.01%,RSD = 2.95%(n = 9).Conclusion The HPLC method is simple,rapid and accurate for the determination of dexamethasone in Compound Lomefloxacin Ear Drops.