可溶性gp130(sgp130)在IL-6/IL-6R系统中的生物学作用研究

Study of biological effect of soluble gp130(sgp130)molecule on the system of interlukin 6/interlukin 6 recepter

目的：在成功克隆、表达和纯化人可溶性ｇｐ１３０（ｓｇｐ１３０）分子的基础上，研究ｓｇｐ１３０在白介素６（ＩＬ６）／白介素６受体（ＩＬ６Ｒ）系统中的生物学作用。方法：采用转人膜型ｇｐ１３０的ＢＡＦ１３０细胞和人多发性骨髓瘤（ＭＭ）细胞株ＸＧ４ＣＮＴＦ和分离得到新鲜ＭＭ细胞，通过３ＨＴｄＲ的掺入做生物学检测，观察ｓｇｐ１３０在上述细胞上对ＩＬ６生物学活性的影响。结果：ｓｇｐ１３０对ＩＬ６和可溶性ＩＬ６Ｒ引起ＢＡＦ１３０细胞的增殖有抑制作用。此外，ｓｇｐ１３０对ＩＬ６刺激ＸＧ４ＣＮＴＦ细胞和分离得到新鲜ＭＭ细胞的增殖也有抑制作用。结论：ｓｇｐ１３０为ＩＬ６的拮抗剂。

Objective:Study of the biological effect of soluble gp130(sgp130)molecule on the system of interlukin 6(IL 6)/IL 6 recepter on the basis of successful cloning,expression and purification of sgp130 molecule.Methods:The membrane gp130 transduced cell line BAF 130,human multiple myeloma(MM)cell line XG4 CNTF and freshly isolated MM cells were used for biological assa by means of 3HTdR incorporation in order to observe biological effect of sgp130 on IL 6 activity to these cells.Results:The results demonstrated that sgp130 displayed inhibitory role in the proliferation of BAF 130 cell line induced by IL 6 and soluble IL 6 recepter.In addition,sgp130 also showed the inhibitory effects on the proliferation,of XG4 CNTF cell lne stimulated by IL 6 and freshly isolated MM cells.Conclusion:These results suggested that sgp130 was an antagonist of IL 6.