摘要
目的:在成功克隆、表达和纯化人可溶性gp130(sgp130)分子的基础上,研究sgp130在白介素6(IL6)/白介素6受体(IL6R)系统中的生物学作用。方法:采用转人膜型gp130的BAF130细胞和人多发性骨髓瘤(MM)细胞株XG4CNTF和分离得到新鲜MM细胞,通过3HTdR的掺入做生物学检测,观察sgp130在上述细胞上对IL6生物学活性的影响。结果:sgp130对IL6和可溶性IL6R引起BAF130细胞的增殖有抑制作用。此外,sgp130对IL6刺激XG4CNTF细胞和分离得到新鲜MM细胞的增殖也有抑制作用。结论:sgp130为IL6的拮抗剂。
Objective:Study of the
biological effect of soluble gp130(sgp130)molecule on the system of interlukin 6(IL 6)/IL 6
recepter on the basis of successful cloning,expression and purification of sgp130
molecule.Methods:The membrane gp130 transduced cell line BAF 130,human multiple
myeloma(MM)cell line XG4 CNTF and freshly isolated MM cells were used for biological assa
by means of 3HTdR incorporation in order to observe biological effect of sgp130 on IL 6
activity to these cells.Results:The results demonstrated that sgp130 displayed inhibitory role in
the proliferation of BAF 130 cell line induced by IL 6 and soluble IL 6 recepter.In
addition,sgp130 also showed the inhibitory effects on the proliferation,of XG4 CNTF cell lne
stimulated by IL 6 and freshly isolated MM cells.Conclusion:These results suggested that
sgp130 was an antagonist of IL 6.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第1期7-9,共3页
Chinese Journal of Immunology
基金
国家自然科学基金
国家教委留学回国人员基金