雷公藤碱戊对系统性红斑狼疮患者B细胞免疫功能的体外实验研究

The study of the effect of Wilforidine on SLE patients'B cell simmunofunction in vitro

目的：探讨雷公藤碱戊的免疫药理作用。方法：采用系统性红斑狼疮（ＳＬＥ）病人外周血单个核细胞（ＰＢＭＣ）体外培养观察雷公藤碱戊对ＳＬＥ病人ＰＢＭＣ的Ｂ细胞功能的影响及时效关系。结果：雷公藤碱戊能抑制ＳＬＥ的ＰＢＭＣ自发性增殖反应以及正常人和ＳＬＥ病人ＰＢＭＣ对ＳＡＣ刺激后的增殖反应。同时在培养的７２ｈ前加入雷公藤碱戊均能对正常人ＰＢＭＣ在ＰＷＭ诱导下的ＩｇＧ产生呈现抑制效应。结论：雷公藤碱戊对Ｂ细胞功能的多个环节具有抑制作用。

Objective:The authors attempted to study wilforidine's immunopharmacologic effect on the SLE patients.Methods:Adopted SLE patients peripheral blood mononuclear cells(PBMC)cultured in vitro and investigated the influence of Wilforidine on PBMC's B cell functions and the time-effect correlation of Wilforidine on PBMC's IgG production induced by PWM.Results:Wilforidine not only inhibited the spontaneous proliferation of PBMC's from SLE patients but also inhibited the proliferation of the PBMC induced by SAC.It could inhibit SLE patients PBMC's IgG production induced by PWM. The time-effect results showed that the Wilforidine had obvious inhibition effect on IgG production when added the drug before 72 hour of the culture time in vitro.Conclusion:These data suggested that Wilforidine could inhibited various B cell's function.The main points of inhibition effect on B cell's IgG production may be on the stage of B cells activation and proliferation.