缬草油对高胆固醇血症大鼠肾小管上皮细胞巢蛋白表达的影响

Effect of Valerian Oil on Expression of Nestin in Renal Tubular Epithelial Cells in Hypercholesterolemic Rats

目的:探讨缬草油对高胆固醇血症大鼠肾小管上皮细胞巢蛋白表达的影响。方法:大鼠随机分为正常组、高脂组、缬草油(25mg·kg-1.d-1)组和辛伐他汀(5mg·kg-1.d-1)组,用含4%胆固醇和1%胆酸钠的高脂饲料饲喂大鼠建立高脂模型。观察8、12、16周时各组大鼠血脂、尿蛋白、血肌酐和肾小管间质病理改变,免疫组化法检测肾小管上皮细胞巢蛋白和α-平滑肌肌动蛋白(α-SMA)表达。结果:随时间延长,高脂大鼠肾小管上皮细胞逐渐出现巢蛋白和α-SMA表达。巢蛋白表达与总胆固醇、低密度脂蛋白、24h尿蛋白、血肌酐正相关(r=0.963、0.830、0.944、0.706,P<0.01),与肾小管间质损伤指数正相关(r=0.974,P<0.01),与α-SMA呈正相关(r=0.804,P<0.01)。缬草油能显著降低大鼠血总胆固醇、低密度脂蛋白、24h尿蛋白和血肌酐,下调肾小管上皮细胞巢蛋白和α-SMA表达(P<0.01)。在巢蛋白表达减少同时,肾小管间质损伤和纤维化明显改善,其改善作用较辛伐他汀更明显(P<0.05)。结论:缬草油可能通过降脂、下调肾小管上皮细胞巢蛋白表达减轻高胆固醇血症大鼠肾间质纤维化。

Objective：To explore the effect of valerian oil on expression of nestin in renal tubular epithelial cells in Hypercholesterolemic rats.Methods：SD rats were randomly divided into the control group,the hyperlipidemia group,the valerian oil group （25 mg·kg-1·d-1 for lavage） and the simvastatin group （5 mg·kg-1·d-1）.Dietary-induced hyperlipidemia were established by given 4% cholesterol and 1% cholic acid diet for 16 weeks.Changes of serum lipid,urinary albumin,serum creatinine and renal interstitial pathological changes were assessed at the 8th,12th and 16th week,and the expressions of nestin protein were detected by immunohistochemical stain.Results：As time elapsed,the expression of nestin and α-SMA in renal tubular epithelial cells were increased significantly.There was positive correlation among the expression of nestin and total cholesterol,low density lipoprotein,urinary albumin and serum creatinine（r=0.963、0.830、0.944、0.706,P0.01）.Nestin also had a positive correlation with tubular-interstitial index（r=0.974,P0.01）and α-SMA（r=0.804,P0.01）.The serum levels of total cholesterol,low density lipoprotein,urinary albumin and serum creatinine were significantly decreased in the valerian oil group than that in hyperlipidemia group,accompanied with reduced expression of nestin and α-SMA in renal tubular epithelial cells（P0.01）.Followed the decreased nestin expression,the renal interstitial injury and fibrosis were relieved significantly in the valerian oil group than in the hyperlipidemia group.The effect was better than that in the simvastatin group（P0.05）.Conclusion：It is concluded that valerian oil has the protective effects on renal interstitial fibrosis by decreasing serum lipid,down-regulation the expression of nestin protein in Hypercholesterolemic rats.