^(125)I放射性粒子植入对犬肠壁影响的病理学观察

The effect of interstitial ^(125)I seeds implantation on intestinal wall:a pathological observation in experimental dogs

目的观察125I放射性粒子对犬肠壁的损伤情况。方法健康成年雄性杂种犬12只为实验对象,随机分为4组,每组3只。实验组将125I放射性粒子(活度0.8mCi)2枚对称植入犬小肠浆膜下,分别饲养14d,1、2个月,观察犬一般情况,到期开腹,取出相应段肠管,作HE染色及电镜检查,观察肠壁组织学变化。结果 125I植入后14d肠管无明显变化,1个月时在电镜下可见细胞损伤,2个月时可见肠壁部分纤维化,无溃疡及穿孔。结论 125I放射性粒子可引起犬肠壁细胞可逆性损伤,术后1个月变化最明显,肠壁部分纤维增生,但不引起溃疡、穿孔等严重并发症。

Objective To observe the radiation injury of the bowel wall due to the implantation of interstitial 125I seeds in experimental dogs.Methods A total of 12 healthy male dogs were randomly and equally divided into 3 experimental groups and 1 control group,with 3 dogs in each group.In the experimental groups,two 125I seeds with the active radiation dose of 0.8mCi were symmetrically implanted under the serous membrane of the dog＇s small intestinal wall.The dogs were fed for 14 days （group A）,for one month （group B） and for two months （group C） respectively when the animals were scheduled to be sacrificed.The dogs＇ general condition was observed till they were sacrificed.The seed-implanting intestinal segments were then removed and dyed with HE staining method for electronic microscopic exam.The histopathologic findings were recorded and the results were compared between four groups.Results No obvious histopathological changes were found in the dog＇s bowel wall 14 days after the implantation.One month after the procedure cellular injury was observed under electronic microscope,and two months after the operation partial fibrosis of the intestinal wall appeared but no ulceration or perforation occurred.Conclusion The implantation of 125I seeds can cause reversible cellular injuries of the intestinal wall in experimental dogs,the degree of the damage reaches its peak at one month after the implant when the partial fibrosis of bowel wall becomes evident.However,the seeds do not cause any serious complications,such as ulceration or perforation.