圆齿野鸦椿叶片的植株再生及快速繁殖

Plantlet regeneration of Eascaphis konlshli leaf and rapid propagation

以圆齿野鸦椿茎段继代繁殖或种子继代繁殖的幼嫩叶片为材料进行组培育苗试验,建立叶片植株再生和快速繁殖的技术体系.结果表明:叶片外植体在WPM+1.0 mg.L-1N6-异戊烯基腺嘌呤(2ip)+1.0 mg.L-1NAA+20 g.L-1蔗糖+7.5 g.L-1琼脂的培养基上形成黄绿色的愈伤组织,30 d后愈伤组织诱导率达95.7%;丛生芽的增殖系数随2ip和NAA含量的增加而加大,2ip含量为1.5 mg.L-1,NAA含量为0.3 mg.L-1时,增殖系数达3.5,WPM+2.0 mg.L-12ip+0.3 mg.L-1NAA+20 g.L-1蔗糖+7.5 g.L-1琼脂是最适的增殖培养基;不定芽在1/2WPM+0.5 mg.L-1IBA+0.3 mg.L-1NAA+20 g.L-1蔗糖+7.5 g.L-1琼脂的培养基上生根效果较好,再生植株移栽的成活率达90%以上.

The tissue culture experiment was conducted with the material of young leaves of stem sect subculture or seed subculture of Eascaphis konlshli Hayata to develop technical system of E.konlshli plantlet regeneration and rapid propagation.The results showed that yellow green calli could be formed from 95.7% leaf explants cultured in vitro for 30 days on WPM medium containing 1.0 mg·L-1 2ip and 1.0 mg·L-1 NAA.Bud multiplication coefficient increased with the increase of concentration of 2ip and NAA,when they separately reached 1.5 mg·L-1 and 0.3 mg·L-1,multiplication coefficient was 3.5.WPM medium supplemented with 2.0 mg·L-1 2ip and 0.3 mg·L-1 NAA was optimum proliferation medium.Adventitious bud has a better rooting result and formed regenerated plants on 1/2WPM medium supplemented with 0.5 mg·L-1 IBA and 0.3 mg·L-1 NAA.The survival rates of regenerated plants were more than 90%.