以DsRed2基因为可视标记的双T-DNA共转化载体的构建

Construction of double T-DNA co-transformation vector with DsRed2 gene as a visual marker

本研究构建了以红色荧光蛋白基因DsRed2作为可视标记的双T-DNA共转化载体PCDMAR-DsRed2-hpt.该载体含有2个独立的T-DNA结构区,其中一个T-DNA区同时含有选择标记基因hpt和红色荧光蛋白基因DsRed2;另一个T-DNA区含有一个通用的多克隆位点,可任意插入目的基因,在多克隆位点两端含有两段顺式重复的烟草Rb7MARs,用来增强目的基因的稳定表达.此载体可用于高效培育无选择标记的转基因植物.

A double T-DNA co-transformation vector PCDMAR-DsRed2-hpt including DsRed2 gene as a visual marker was contructed,which was composed of two tranfering DNA region.One of T-DNA region includes selecting marker gene hpt and red fluorescent protein DsRed2.The other T-DNA region includes a universal multiple cloning sites for inserting target genes.Two pieces of directrepeat tabacoo Rb7MAR sequence flanked the multiple cloning sites to increase target stable expression.The above newly created plant expression vectors can be used to develop marker-free transgenic plants efficiently for a variety of purposes.