体外抑菌实验微量定量检测方法的建立与探索

Inhibition of germ by microquality and quantity analysis

目的：介绍微量定性定量法（ＭＱＱＡ），为抗菌药物的筛选和药敏试验提供一种新的方法。方法：以大肠杆菌、绿脓杆菌和金葡球菌为实验对象，以硫酸丁胺卡那霉素、庆大霉素２个已知药为工具药，用ＭＱＱＡ测出２个已知药对上述３个菌株的最小抑菌浓度（ＭＩＣ），并与平皿法和试管法进行比较。结果：ＭＱＱＡ的ＭＩＣ值对细菌活性的影响，通过酶标仪测试其吸光度值，求出ＥＤ５０。结论：ＭＱＱＡ具有重要的应用价值。

OBJECTIVE:To find the better way of inhibition of germ.METHODS:The way of inhibition of Escherichia coli,Bacillus pyocyaneus,Staphylococcus aureus by amicacine and gintamincine,the methods of the plate,the tube and U type disk (quantitative analysis by microdose and germ MQQA) were compared in nathre.RESULTS:Minimun inhibitory concentration(MIC) by MQQA was lower than that by other two methods with higher sensitivity.MQQA was benifitial action of drug on germ in quantitative analysis by the reagent MTT and ED 50 (median effective dose) could be counted by the result. CONCLUSION:MQQA is important in researching antibacteria drugs and testing drug sensitivity on germs.