色氨酸羟化酶2基因多态性与重症抑郁发作和治疗反应的关系分析

Association of tryptophan hydroxylase 2 gene polymorphisms with major depression disorder and antidepressant treatment response

目的分析中国汉族人群中色氨酸羟化酶2(TPH-2)基因单核苷酸多态性(SNP)rs4570625和rs7305115位点与重症抑郁发作(MDD)和抗抑郁药物治疗反应的关系。方法设立病例组(汉族MDD患者,n=346)和对照组(汉族正常人,n=347);根据对治疗的反应,病例组患者再分为难治性抑郁症组(TRD组,n=72)和非TRD组(NTRD组,n=274)。通过Taqm an探针SNP基因分型技术对TPH-2基因的rs4570625和rs7305115位点进行基因分型,分析等位基因和基因型频率在各组间的分布差异。结果病例组TPH-2基因rs4570625位点的基因型及等位基因分布的频率与对照组比较,差异有统计学意义(P<0.05)。按性别分层后,男性TRD组与男性NTRD组SNPs rs4570625和rs7305115位点的基因型和等位基因分布频率比较,差异有统计学意义(P<0.05)。结论 TPH-2基因rs4570625位点可能与抑郁症的发病有关;男性患者rs4570625和rs7305115位点的多态可能可用于预测对治疗的反应。

Objective To investigate the association of tryptophan hydroxylase 2（TPH-2） gene single nucleotide polymorphisms（SNPs） of loci rs4570625 and rs7305115 with major depression disorder（MDD） and antidepressant treatment response in Chinese Han populations.MethodsA total of 346 Chinese Han patients with MDD（case group） and 347 healthy Chinese Han people（control group） were enrolled,and patients in case group were subdivided into treatment-resistant depression（TRD） group（n=72） and non-TRD（NTRD） group（n=274） according to treatment response.SNPs of rs4570625 and rs7305115 were selected for the genomic region of TPH-2 gene and genotyped through TaqMan SNP genotyping assay,and the distribution of gene type and allele in groups was analysed.ResultsThere were significant differences in distribution of rs4570625 gene type and allele between case group and control group（P0.05）.There were significant differences in distribution of SNPs rs4570625 and rs7305115 gene type and allele between males of TRD group and males of NTRD group（P0.05）.ConclusionTPH-2 gene rs4570625 may be associated with depression disorder in Chinese Han populations,and SNPs of rs4570625 and rs7305115 may predict the antidepressant treatment response in male patients.