氨甲酰-妥布霉素产生菌的高产菌株筛选

The Screening of High Yielding Carbamoyltobramycin Strains

以黑暗链霉菌Ｓｔｒｅｐｔｏｍｙｃｅｔｅｎｅｂｒａｒｉｕｓ１６３为出发菌株，应用磁场、磁场复合吖啶橙、耐受氨甲酰妥布霉素、磁场复合耐受氨甲酰妥布霉素筛选得到５株高产菌株，经摇瓶发酵复筛后表明。

A tobramycin producing strain, Strepomyces tenebrarius 163 was mutated by magnetic field,magnetic field combined with acriding and resisted by carbamoyltobramycin,carbamoyltobramycin combined with magnetic field.Several high yielding mutants were screened out.After flask rescreening,it is indicated that these mutants′ potency and the percentage of carbamoyltobramycin increased by above 25% than those of the parent strain.