摘要
目的建立近交系大鼠穿透性角膜移植动物模型,观察结膜下注射FK506对角膜移植免疫排斥反应的抑制作用。方法将近交系Lou大鼠28只作受体,14只F344大鼠作供体,分为3组,术后结膜下分别按每公斤体重注射0.1mgFK506、3mgCsA及生理盐水,共2周。对角膜植片进行临床观察,以混浊、水肿和新生血管3项指标作为临床评估标准。结果3组角膜植片的存活时间分别为(221±517)、(184±14)及(121±213)天,三者间差异有显著性(P<0.01)。结论FK506能显著延长大鼠角膜植片的存活时间,是一种有效的新型免疫抑制剂。
Objective To study the immunosuppressive effects of FK 506 on allogeneic transplantation in a rat model. Methods Inbred strain Lou rats were used as recipients, and F344 rats were used as donors. Subconjunctival injection of FK 506 0.1 mg/kg and cyclosporine A (CsA) 3 mg/kg were administered respectively for 2 weeks, and the grafts were inspected by clinical evaluation. Results The group receiving FK 506 had a significant delay in allograft rejection with mean survival time (22.1±5.17) days vs. (18.4±1.4) days for the CsA group and (12.1±2.13) days for the control group ( P <0.01). Conclusion It is indicated that FK 506 would be a useful drug to suppress corneal graft rejection.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1999年第1期25-28,共4页
Chinese Journal of Ophthalmology