FK-506抑制大鼠角膜移植免疫排斥反应的研究

Experimental studies on the effect of the immunosuppressant FK 506 on penetrating keratoplasty rejection model in rats

目的建立近交系大鼠穿透性角膜移植动物模型，观察结膜下注射ＦＫ５０６对角膜移植免疫排斥反应的抑制作用。方法将近交系Ｌｏｕ大鼠２８只作受体，１４只Ｆ３４４大鼠作供体，分为３组，术后结膜下分别按每公斤体重注射０．１ｍｇＦＫ５０６、３ｍｇＣｓＡ及生理盐水，共２周。对角膜植片进行临床观察，以混浊、水肿和新生血管３项指标作为临床评估标准。结果３组角膜植片的存活时间分别为（２２１±５１７）、（１８４±１４）及（１２１±２１３）天，三者间差异有显著性（Ｐ＜０．０１）。结论ＦＫ５０６能显著延长大鼠角膜植片的存活时间，是一种有效的新型免疫抑制剂。

Objective To study the immunosuppressive effects of FK 506 on allogeneic transplantation in a rat model. Methods Inbred strain Lou rats were used as recipients, and F344 rats were used as donors. Subconjunctival injection of FK 506 0.1 mg/kg and cyclosporine A (CsA) 3 mg/kg were administered respectively for 2 weeks, and the grafts were inspected by clinical evaluation. Results The group receiving FK 506 had a significant delay in allograft rejection with mean survival time (22.1±5.17) days vs. (18.4±1.4) days for the CsA group and (12.1±2.13) days for the control group ( P <0.01). Conclusion It is indicated that FK 506 would be a useful drug to suppress corneal graft rejection.