摘要
gp130是一种分子量为130kD的细胞膜糖蛋白,是IL-6等多种细胞因子共用的信号传递分子。本研究将含gp130胞外区全长的可溶性片段的基因(sgp130cDNA)构建到痘苗病毒载体中,并与野生型痘苗病毒在TK-143细胞中发生同源重组,用BudR和X-gal双重选择,得到带有人sgp130的重组痘苗病毒(Vsgp130)。采用IDA和WesternBloting法证实:感染Vsgp130的TK-143细胞上清中有较强的sgp130表达,表达产物分子量为100kD左右。
gp130 is a
cell membrane glycoprotein of 130 kDa.Some cytokines such as IL 6,ciliary neurotro
phic factor(CNTF),oncostatin M(OM)and leukemia inhibitory factor(LIF) share the gp130 as
a common signal transducer.We have constructed soluble gp130(sgp130) recombinant
expression vector by inserting the sgp130 cDNA into the vaccinia virus expression
plasmid pJSA1175.This recombinant plasmid was then used for homologous
recombination with the wild type vaccinia virus genome in TK -143 cells.sgp130
recombinant vaccinia virus(Vsgp130) was then obtained by the BudR and X gal double
sellections.Immunodot assay and Western blotting analysis showed that the recombinant
vaccinia virus could express sgp130 into the supernatant of TK -143 cells and the
molecular weight of expression product was about 100 kDa.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1999年第1期27-29,共3页
Immunological Journal