IL-2在体内抗弓形虫作用机制的研究

THE EFFECTS OF IL 2 AGAINST TOXOPLASMA GONDII INFECTION IN VIVO

为观察ＩＬ－２在小鼠体内抗弓形虫中的作用，应用３Ｈ－尿嘧啶（３Ｈ－Ｕ）特异性标记的弓形虫在小鼠腹腔巨噬细胞（Ｍｏｕｓｅｐｅｒｉｔｏｎｅａｌｍａｃｒｏｐｈａｇｅ，ＭＰＭ）内的增殖实验及１２５Ｉ－尿嘧啶核苷（１２５Ｉ－ＵｄＲ）标记的细胞毒实验，观察ＩＬ－２的抗弓形虫作用。结果：ＩＬ－２对ＭＰＭ的抗弓形虫作用及对ＩＦＮ－γ诱导的ＭＰＭ抗弓形虫作用无明显影响；ＩＬ－２体内应用可明显增强小鼠抗急性弓形虫感染的能力，延长小鼠生存时间；ＩＬ－２治疗组小鼠脾细胞杀伤肿瘤细胞及杀伤弓形虫自身感染靶细胞的能力，均明显高于对照组；同时也发现，对照组小鼠脾细胞几乎不能杀伤弓形虫感染的自体靶细胞。提示ＩＬ－２体内应用的抗弓形虫机制在于提高机体免疫细胞杀伤弓形虫感染细胞的能力。

In vitro,IL 2 did not induce the toxoplasmastatic activity of mouse peritoneal macrophage(MPM)combining with various concentrations of IFN γ,IL 2 did not enhance these effector functions more than IFN γ alone.However in vivo 700U IL 2 could made mice prolonge survival time compared with control mice after infection with 100 tachyzoites of RH strain( P <0.05).Studies on the protective mechanism demonstrated that IL 2 could significantly enhance the NK and LAK activity to kill tumor target cells and LAK activity to kill autologous spleen cells infected with Toxoplasma gondii but not NK activity.