豚鼠血清功能纯C2分子的制备和鉴定

PREPARATION AND IDENTIFICATION OF THE FUNCTIONA LLY PURE C2 IN GUINEA PIG SERUM

以低渗沉淀方法制备的豚鼠血清功能纯Ｃ１及人血清为实验材料，建立Ｃ２溶血活性检测方法，经ＣＭ－ＳｅｐｈａｄｅｘＣ５０离子交换色谱分离纯化豚鼠血清功能纯Ｃ２分子。经鉴定，所得功能纯Ｃ２具有良好活性，无Ｃ１及Ｃ３～Ｃ９活性成份，可用于体外组装经典途径Ｃ３转化酶。本工作为进一步研究经典途径Ｃ３转化酶的补体调控蛋白奠定了基础。

Haemolytic assays for the second component of complement was established by using human fresh serum and functionally pure C1 gp prepared by euglobulin precipitatoin. Functionally pure C2 without C1 and C3～C9 activity was prepared by ion exchange chromatography on CM Sephadex C50. Assembly of the classical pathway C3 convertase in vitro using functionally pure gp C1, gp C2 and EAC4 hu enable us to further investigate the function of complement regulatory proteins.