巴西橡胶树磷转运蛋白基因的克隆及生物信息学分析

Molecular Cloning and Bioinformatic Analysis of a Gene Encoding a Phosphate Transporter from Hevea brasiliensis

植物磷转运子Pht1家族是一类H2PO4-/H+共转运子,该家族主要成员在植物根系中负责磷的吸收和转运,其表达受磷调控,对该家族成员的研究有助于揭示磷的吸收和转运机制。根据Pht1家族基因的保守性设计简并引物,利用RT-PCR和RACE技术从巴西橡胶树(Hevea brasiliensis)中克隆了1个Pht1基因,命名为HbPht1。氨基酸序列同源性比对与系统进化分析表明,HbPht1属于磷转运蛋白基因家族的新成员。该基因的克隆和生物信息学分析为进一步研究其功能奠定了基础。

The Pht1 family of phosphate transporters in plants is a type of H2PO4-/H＋ symporter, which is in charge of absorbing and transporting Pi in plant root.The expression of Pht1 family is regulated by Pi, whereas studying the members of Pht1 family will be helpful to elucidate the mechanisms of absorbing and transporting Pi.According to the conservative evolution of plant Pht1 family, the degenerate primers of Pht1 family in rubber tree were designed.The Pht1 in rubber tree, named as HbPht1, was cloned by RT-PCR and RACE techniques.Amino acid sequence alignment and phylogenetic analysis revealed that HbPht1 was a new member of the phosphate transporters family.This paper will be benefit to further analyze the functions of HbPht1.