骨形成发生蛋白4和SMAD4在胶质瘤诊断中的意义

Significance of Bone Morphogenetic Protein 4 and SMAD 4 in the Diagnosis of Glioma

目的探讨骨形成发生蛋白4(BMP4)和SMAD4表达变化在胶质瘤诊断中的意义。方法随机选取胶质瘤标本45例,正常脑组织10例,应用RT-PCR和Western blot检测胶质瘤标本和正常脑组织中BMP4和SMAD4表达。结果 RT-PCR结果显示,与正常脑组织相比,低级别胶质瘤、高级别胶质瘤中BMP4表达分别降低(23.5±0.23)%、(46.6±0.34)%,差异有统计学意义(P<0.05)。SMAD4表达分别降低(30.6±0.41)%、(95.3±1.02)%(P<0.05)。Western blot结果显示与正常脑组织相比,低级别胶质瘤、高级别胶质瘤BMP4表达分别降低(28.4±0.25)%、(80.4±1.05)%(P<0.05)。SMAD4表达降低(76.8±1.15)%、(96.2±2.01)%(P<0.05),差异有统计学意义。结论 BMP4、SMAD4可能与胶质瘤的发生发展及恶性程度相关,BMP4、SMAD4可能成为胶质瘤诊断和分级的一个重要指标。

[Objective]To study the significance of expression changes of bone morphogenetic protein 4 （BMP4） and mothers against decapentaplegic homolog 4 （SMAD4） in the diagnosis of glioma.[Methods]45 samples of glioma and 10 samples of normal brain tissue were collected randomly. The expressions of BMP4 and SMAD4 were detected with RT-PCR and Western blot.[Results]RT-PCR results showed：compared to the normal brain tissue,the expression of BMP4 in low-grade gliomas and high-grade gliomas was reduced by （23.5±0.23） % and （46.6±0.34） %,and the difference was significant （P0.05）. The expression of SMAD4 was reduced by （30.6±0.41） % and （95.3±1.02） % respectively （P0.05）. Western blot results showed：compared to the normal brain tissue,the expression of BMP4 was reduced by （28.4±0.25） % and （80.4±1.05） % respectively （P0.05）,the expression of SMAD4 was reduced by （76.8±1.15） % and （96.2±2.01） % respectively,and the difference was significant （P0.05）.[Conclusion]BMP4 and SMAD4 may be related to the occurrence,development and malignant degree of glioma. BMP4 and SMAD4 can be regarded as an important index in the diagnosis and classification of glioma.