全反式维甲酸诱导人肝癌细胞HepG2凋亡的初步研究

All-trans-retinoic Acid Induced Apoptosis of Human Hepatoma HepG2 Cells

[目的]研究全反式维甲酸(ATRA)体外诱导人肝癌细胞株HepG2的凋亡及其作用机制。[方法]ATRA处理HepG2细胞。MTT法分析细胞增殖反应;Hoechst染色法检测细胞凋亡情况;Western blot检测细胞中caspase-9、caspase-3和NF-κB蛋白表达情况。[结果]A-TRA可抑制HepG2细胞增殖并诱导细胞凋亡,可上调细胞中caspase-9、caspase-3表达水平(P<0.05)。[结论]ATRA可抑制HepG2细胞增殖并诱导细胞凋亡,其作用机制可能与cas-pase-9、caspase-3表达上调有关。

[Purpose] To investigate all-trans-retinoic acid （ATRA） induced apoptosis of human hepatoma HepG2 cells in vitro and its mechanism.[Methods] HepG2 cells line pretreated with ATRA.The proliferation of HepG2 was analyzed by MTT assay.The cell apoptosis was measured by Hoechst dye.The expressions of caspase-9,caspase-3 and NF-κB protein were detected by Western blot.[Results] ATRA could inhibit proliferation and induce apoptosis of HepG2 cell,Moreover,it could up-regulated caspase-9,caspase-3 expression level.[Conclusion] ATRA could inhibit proliferation and induce apoptosis of HepG2 cells and this effect maybe associated with upregulation of caspase-9 and caspase-3 expression.