三种血小板抗体检测方法的临床应用比较

Compare Three Methods of Platelet Antibody Detection and Its Clinical Application

目的比较ELISA法、传统的淋巴细胞毒性试验（CDC）和流式细胞技术（FCM）检测血小板抗体，确定一种准确、简便、稳定的试验方法。方法用三种方法分别对50例血小板减少性紫癜患者（与血小板抗体相关）和50例正常人作为对照进行血小板抗体检测，验证这三种方法检测血小板抗体的灵敏度和特异度，并比较各种方法之间的优点和不足。结果ELISA方法检测血小板抗体的方法操作简单，且其准确度和特异度均与流式细胞法相近，差异无统计学意义（P〉0．05），二者检测效果均优于淋巴毒性试验的方法（P〈0．05）。结论ELISA法检测血小板抗体的方法具有准确度和特异性高而且操作简单易于推广的特点。

Objective To compare ELISA,the method with the traditional lymphocyte toxicity test （CDC） and Flow cytometry （FCM） method,to investigate the differences of them,the aim was to establish an accurate,simple and stable method of platelet antibodies detection. Methods The three methods were employed to detect the platelet antibodies of 50 cases of idiopathic thrombocytopenic purpura patients （platelet antibody-associated） and 50 normal persons as controls,the sensitivity and specificity of the three methods were verified ,and the strengths and weaknesses of them were analysed. Results The elisa method of platelet antibody detection was simple to perform,and its accuracy and specificity were similar to flow cytometry method, the difference between them was not statistically （P〉 0.05）. The detection effects of the two methods were better than that of the traditional lymphocyte toxicity test （CDC） method （P〈0. 05）. Conclusion The Elisa method of platelet antibodies detection was of high accuracy and specificity and is simple to perform and easy to promote.