Insulin/FoxO1/Pdx1/MafA基因在宫内发育迟缓大鼠胰腺发育中的表达

Gene expression of Insulin/FoxO1/Pdx1/MafA during pancreatic development in intrauterine growth retardation rats

目的:探讨宫内发育迟缓(intrauterine growth retardation,IUGR)大鼠在胚胎期和新生后Insulin/FoxO1/Pdx1/MafA基因的表达。方法:清洁级SD大鼠,雌雄鼠1∶3合笼交配,孕鼠按受孕顺序随机分为2组(IUGR组和对照组)。IUGR组自妊娠第1天至分娩给予正常对照组饲料进食量的半量;对照组妊娠全程任意摄取饲料;应用显微分离及提取技术取大鼠胚胎发育第14.5天、第19.5天及新生大鼠胰腺组织;采用HE染色,光镜观察胰腺不同发育时期形态学变化;使用RT-PCR技术检测胚胎发育第14.5天,第19.5天及新生大鼠胰腺Insulin/FoxO1/Pdx1/MafA基因的表达情况。结果:①与对照组大鼠相比,IUGR组胰腺在发育各期均有形态学上的改变,表现为组织松散,结构不清晰,胰岛数量及面积减少;②Insulin/FoxO1/Pdx1/MafA基因全程均有表达;③Insulin、MafA基因随胎龄的增长表达量增多,与对照组相比,IUGR组Insulin基因表达无明显差异(P>0.05),而MafA基因表达较之减少(P<0.05);④FoxO1、Pdx1基因均在胚胎早期表达较多,随胎龄的增长表达量下降,且与对照组相比,IUGR组Pdx1基因表达无明显差异(P>0.05),FoxO1表达较对照组减少(P<0.05)。结论:宫内发育迟缓对胰腺发育相关基因Insulin/FoxO1/Pdx1/MafA的表达有影响。

Objective：To investigate the expression of Insulin/FoxO1/Pdx1/MafA genes during pancreatic development in the intrauterine growth retardation（IUGR）rats.Methods：①The establishment of rat model：female and male SD rats were copulated by 1：3,and then were divided into the following two groups：IUGR group and normal group.The former were treated by 50% calorie compared with normal from gestational day 1 until term.②The collection of samples：pancreas of rat embryonic day14.5（E14.5）,19.5（E19.5）and new born were dissected under microscope respectively.③The detection of pancreatic morphological changes：HE staining and light microscope were used.④The expression of Insulin/FoxO1/Pdx1/MafA genes：after abstraction of total RNA,the technique of RT-PCR was used to detect the expression of Insulin/FoxO1/Pdx1/MafA genes.Results：①Compared with normal,the pancreatic construction,islet area and quantities had abnormal morphological changes during pancreatic development.②The genes of Insulin/FoxO1/Pdx1/MafA began to be expressed from E14.5,and the expressions of Insulin and MafA were increased as the fetus grew while FoxO1 and Pdx1 were decreased.③Compared with the normal,the expressions of FoxO1 and MafA were lower in IUGR rats（P0.05）,while Insulin and Pdx1 had no evident change.Conclusion：The expressions of Insulin/FoxO1/Pdx1/MafA genes were affected by intrauterine growth retardation.