甲状腺激素对急性肝衰竭大鼠肝细胞的保护作用

Protective effect of thyroid hormone on the hepatocytes in a rat model of acute hepatic failure

目的:探讨补充外源性甲状腺激素对急性肝衰竭大鼠肝细胞的保护作用。方法:将SD大鼠36只随机分为3组:对照组、急性肝衰竭组和治疗组。利用D-半乳糖胺腹腔注射制作大鼠急性肝衰竭模型,观测是否存在正常甲状腺病态综合征;通过腹腔注入10mg/L三碘甲状腺原氨酸(T3)1.5ml/kg,以纠正肝衰竭大鼠的低T3状态,检测血清生化指标并用光镜观察肝细胞的变化和凋亡。结果:急性肝衰竭大鼠模型存在低T3状态,治疗组大鼠肝衰竭表现轻,48h存活率显著高于急性肝衰竭组(P<0.05)。急性肝衰竭组大鼠肝细胞明显变性坏死,而治疗组肝细胞受损程度较轻。结论:急性肝衰竭大鼠存在正常甲状腺病态综合征,补充外源性甲状腺激素可以改善急性肝衰竭预后,并且对急性肝衰竭时肝细胞有明显的保护作用。

Objective：To explore the protective effect of thyroid hormone supplement on the hepatocytes in a rat model of acute hepatic failure. Methods：Thirty-six SD rats were randomly distributed in three groups. In group-1 （control group）,the rats received only placebo. In group-2 （AHF group）,the rats received intraperitoneal injection of D-galactosamine and saline. In group-3（treatment group）,the rats received intraperitoneal injection of triiodothyronine （T3）at a dose of 15 mg / kg in addition to D-galactosamine. Blood and liver samples were obtained in all animals for the determination of thyroid and hepatic function and assessment of hepatic injuries. Results：T3 was subnormal in rat model of acute hepatic failure. There were less hepatocyte injuries and lower mortality in the treatment group as compared to the AHF group （P 0.05）. Conclusion：A rat model of acute hepatic failure has euthyroid sick syndrome. Thyroid hormone supplement may improve the prognosis and have significant hepatocyte protection in this animal model of acute hepatic failure.