SD大鼠糖尿病肾病模型构建的比较

Comparison different model construction methods of diabetic nephropathy SD rats

目的:探讨不同方法建立糖尿病肾病(diabetic nephropathy,DN)模型的差异,以确定建立糖尿病肾病大鼠模型的最佳方案。方法:清洁级健康雌性SD大鼠30只,随机分为3组,每组10只。A组大鼠单侧肾脏切除后腹腔注射链脲佐菌素(streptozotocin,STZ)55mg/kg,B组大鼠单侧肾脏结扎后腹腔注射STZ55mg/kg,C组为假手术对照组,注射等量的缓冲液。大鼠造模后从第1周开始每2周称体重,取血测血糖(Glu)、血肌酐(Scr)、总胆固醇(Chol)和甘油三酯(TG),收集尿测24h尿蛋白(24Upro)、尿肌酐(Ucr),并计算内生肌酐清除率(Ccr)。11周后,处死大鼠,取肾脏称重并进行肾脏病理。结果:A、B2组的成模周期相当,B组的存活率高于A组。结论:单侧肾脏结扎后再腹腔注射55mg/kgSTZ可以用来作为建立糖尿病肾病大鼠模型的理想方案。

Objective：To investigate the different model construction methods in diabetic nephropathy （DN） SD rats induced by streptozotocin（STZ） and establish the optimal plan. Methods：Thirty SD rats were randomly divided into 3 groups with 10 rats in each group. A group received intraperitoneal STZ after unilateral nephritic excision;B group received intraperitoneal STZ after unilateral nephritic ligation;C group（control group） received buffer solution only. The changes of body weight（BW）,glucose（Glu）,serum creatinine （Scr）,cholesterol（Chol）,triglyceride （TG）and 24-urine protein（24Upro）,urine crea（Ucr）were detected every 2 weeks and creatinine clearance rate（Ccr） was calculated. After 11 weeks,the rats were sacrificed,and the kidneys were weighed and taken for pathological examination. Results：There was no significant difference in the model construction periods between the groups A and B. The survival rate of the group B is higher than that of the group A. Conclusion：STZ （55 mg / kg） intraperitoneal injection after unilateral nephritic ligation is the optimal method for inducing rat diabetic nephropathy.