慢性间歇性低氧对SD幼鼠生长发育影响的实验研究

The influence of chronic episodic hypoxia to the development of juvenile Sprague-Dawley rats.

目的利用间歇性低氧诱导幼年Sprague-Dawley幼鼠,探索间歇性低氧对幼鼠生长发育的影响。方法 20d雄性SD鼠24只,体重(45±4.7)g,随机分成间歇性低氧组(E组)和对照组(C组),各12只。E组行间歇性低氧4周,对照组暴露于空气,分别于第1(T1)、2(T2)、3(T3)、4(T4)周监测两组幼鼠的身长(EL、CL)及体重(EW、CW)。结果①E组、C组在T3、T4时点身长体重明显高于T1(P<0.05)。②在T3、T4时点E组明显低于C组(P<0.05)。结论 SD幼鼠经间歇性低氧诱导后生长缓慢,符合小儿鼾症临床特征,故利用间歇性低氧诱导SD幼鼠,建立动物模型是可行的。

Objective Juvenile Sprague-Dawley（SD）rats were induced by intermittent hypoxia to establish hypoxia animal models.Methods Twenty-four male SD rats,aged 20 days,weighted（45±2.7）g,were randomly divided into intermittent hypoxia（group E,n=12）and control groups（group C,n=12）.Cases in group E were exposed to intermittent hypoxia for 4 weeks,while Cases in group C were exposed to air.Weight and legth were measured at each weekend.Results ①Weight and length at T3and T4 were lower than T1 of that in group E and C（P0.05）.②group E were lower than group C at T3,T4.Conclusion Growth dysfunction in intermittent hypoxia group rats was coincidence with the clinical characteristics of OSAHS children demonstrating it is feasible to establish the animal models by inducing young SD rats with intermittent hypoxia.