万乃洛韦毫微粒对肝脏和肝细胞胞内靶向性的研究

Study on liver and hepotocytes targeting of valaciclovir polybutyl cyanoacrylate nanoparticles

目的：测定静注万乃洛韦聚氰基丙烯酸正丁酯毫微粒（ＶＡＣＶＰＢＣＡＮＰ）冻干剂和万乃洛韦小鼠体内的分布。方法：用体外肝细胞培养及摄取实验研究ＶＡＣＶＰＢＣＡＮＰ对肝细胞的胞内靶向性。结果：ＶＡＣＶＰＢＣＡＮＰ静注后１５ｍｉｎ即有７４．４９％的ＶＡＣＶ集中在肝脏，比ＶＡＣＶ注射液提高了２．９９倍，肾脏分布量降低了５．４６倍。

OBJECTIVE:To study the distribution of VACV PBCA NP in visceras of mice.METHODS:Uptake of hepotocytes was used to study the permeation of VACV PBCA NP cross the liver cell membrane.HPLC was used to determine the contents of ACV in visceras.RESULTS:The content of VACV PBCA NP in liver was found to be 74.79% of total amount 15 min after iv administration,which was 2.99 times higher than value of VACV injection.While content in kidney was 5.46 times lower than that of VACV in injection.The VACV PBCA NP was found in liver cell of mice by TEM method.CONCLUSIONS:The cell uptake by rat hepotocytes in vitro demonstrated that nanoparticles could greatly increase the amount of VACV uptaking by cell.