甲基叔丁基醚对SD大鼠的亚慢性毒性

Subchronic Toxicity of Methyl Tertiary Butyl Ether on SD Rats

目的了解汽油添加剂甲基叔丁基醚（ＭＴＢＥ）对ＳＤ大鼠的亚慢性毒性。方法４０只雄性ＳＤ大鼠，体重１８０至２００ｇ，被随机分为４组。灌胃染毒，染毒剂量分别为１０００、６００、２００和０ｍｇｋｇ体重。每天１次，每周５ｄ，共１３周。染毒结束后对肝、肾、肺及睾丸等主要器官进行病理学观察，检测血清酶和蛋白水平及肝细胞色素Ｐ４５０的含量。结果各组动物未见体重减轻和异常行为改变，除６００及１０００ｍｇｋｇ剂量组动物肝、肾脏器系数明显增加外，未见ＭＴＢＥ对肝肾功能和其他血清酶有明显影响，光镜检查主要脏器也未发现明显病理改变。电镜检查发现，ＭＴＢＥ可引起肝细胞核浓缩，脂滴和溶酶体增多，滑面内质网断裂等。ＭＴＢＥ也可明显诱导大鼠肝细胞色素Ｐ４５０的明显升高。结论ＭＴＢＥ对ＳＤ大鼠具有一定的毒作用，肝细胞色素Ｐ４５０在ＭＴＢＥ的代谢中起着重要作用。

Results MTBE significantly increased the weights of liver, kidney,and hepatic cyrochrome P450 content in all treated groups.Electron microscopy analysis showed significant changes in liver cells of the treated groups,including cell nuclear condensation,fat drops and lysosomes appearance in cells,smooth endoplasmic reticulum (SER) dispersion and liver cell necrosis. Conclusions MTBE may be toxic to the liver of SD rats,and cytochrome P450 may be involved in MTBE metabolism.