巢式RT-PCR检测荷人肝癌裸鼠周围静脉血液中AFPmRNA

Detection of blood AFPmRNA in the mice bearing with human carcinoma using nested reverse transcriptase polymerase reaction

目的应用巢式RT－PCR检测荷人肝癌裸鼠周围静脉血液中AFPmRNA并探讨其意义。方法抽取20只荷人肝癌裸鼠周围静脉血液各1ml，应用巢式RT－PCR检测其周围静脉血液中AFPmRNA。结果20只荷瘤裸鼠中有6只血液中被检测到AFPmRNA（30.0％），其中有4只发现了肺脏、肝脏或肾脏转移（66.67％）。结论AFPmRNA可以作为肝癌血液转移的敏感性指标，也可作为肝癌远处转移的预测性指标之一。

Objective To detect the blood AFPmRNA in the nude mice bearing with hepatocellular carcinoma (HCC) using nested reverse transcriptase polymerase reaction (nested RT-PCR) and its implication. Method One ml of blood each in 20 samples from the nude mice bearing with human HCC was taken and the AFPmRNA was detected by using nested RT-PCR. Result AFPmRNA was detectable in 6 blood samples from mice bearing with human HCC (30. 0 % ), in which 4 mice were found having metastasis (lung, liver, or kidney). Conclusion AFPmRNA may be used as an efficient and sensitive indicator for blood spread of HCC cells and for the occurrence of metastasis of HCC.