Tb-NT-P-BSA抗原在结核病血清诊断的初步评价

EVALUATION OF Tb NT P BSA ANTIGEN IN THE SERODIAGNOSIS OF TUBERCULOSIS

用人工合成的结核杆菌酚糖酯三糖抗原Ｔｂ－ＮＴ－Ｐ－ＢＳＡ作抗原，用ＥＬＩＳＡ法检测了４０例结核患者、５２个健康对照的血清标本。结果表明：本方法的特异性、敏感性分别是：在检测抗Ｔｂ－ＮＴ－Ｐ－ＢＳＡＩｇＧ抗体时为９４．２％和２０％；在检测抗Ｔｂ－ＮＴ－Ｐ－ＢＳＡＩｇＭ抗体时为７９．６％和４５％。该抗原的特异性、敏感性似不理想。

Sera from 40 tuberculous patients and 52 healthy controls were assayed by ELISA using Tb NT P BSA(containing the terminal triglycosyl unit of the M.tuberculosis phenolic glycolipid)as antigen.The sensitivity and specificity were 94.2%,20% for IgG and 79.6%,45% for IgM antibody activity resperctively.The results show that the antigen is not sensitive.That will limit its use in the serodiagnosis of tuberculosis.