32种果树microRNA的生物信息学预测与分析

Computational Identification and Analysis of the Putative microRNAs in 32 Fruit Crops

通过生物信息学预测miRNA的方法,采用基于生物信息学的基因搜索和同源搜索的方法成功地从NCBI数据库中登录的32种果树的EST中寻找miRNAs。从32种果树的774400条ESTs中找到了110条miRNA前体序列,编码116条成熟体序列。利用miRbase数据库进一步分析,结果表明116个miRNAs属于45个miRNA家族,其中有7个保守的miRNA家族中的miRNA个数在5以上,20个miRNA家族的miRNA个数在2～4,有18个miRNA家族的miRNA数量为1个。在柑橘、苹果、香蕉、猕猴桃和桃等果树中分别发现28、16、13、11和10个miRNAs。果树中miRNA的序列比较发现,相同家族的miRNA的序列存在一定水平的差异,其中碱基的变化包括相似频率的颠换与转换。

Here we present a study of bioinformatics identification of microRNA precursors in fruit crops by blasting ESTs of 32 fruit crops in NCBI using bioinformatics-based gene search and homology search method to look for miRNAs in fruit crops.A total of 110 miRNA precursors,encoding 116 unique miRNA sequences,were identified in these fruit crops.Further analysis through the miRbase database showed the 116 miRNAs belonged to 45 miRNA families,of which 7 were predicted with miRNA number more than 5,20 between 2–4,and the other 18 with only 1 miRNA.miRNAs were identified in Citrus,apple,bananas,kiwifruit and peach,and the numbers of miRNAs found in them were 28,16,13,11,and 10,respectively.The results of the alignment of 6 miRNA families showed the nucleotide sequences of the miRNAs in same family were variable,and the variation between the nucleotides of same locus could be of transversion and transition.