甘蓝细胞质雄性不育材料分子鉴定及花器官形态对核背景的响应

Molecular Identification of Brassica oleracea CMS and the Morphology Response of Flower to Nuclear Background

对从国内外引进的15份甘蓝细胞质不育材料进行分子鉴定,并通过多代连续回交转育,研究转育后代花器官形态对核背景的响应。结果发现,15份不育材料中有14份为萝卜胞质不育(Ogu CMS),仅1份为甘蓝型油菜胞质不育(Nap CMS)。细胞质不育材料花器官对核背景的响应,Nap型主要表现3种类型,第1类与原材料相近或呈负响应,第2类表现为正响应,第3类花器官不同部位响应不一,呈现正或负响应;Ogu型主要表现为不变化或较弱的正响应。不同来源胞质材料的花器形态对同一核背景的响应,因自交系不同而异,对于自交系K1、N4,不同来源胞质材料对其响应趋于一致,对自交系37、F1、G7的响应,在不同来源胞质材料间存在差异。

Fifteen cytoplasmic male sterile materials in Brassica oleracea was identified by amplified special cytoplasmic male sterile associated gene,all of the CMS materials were crossed and backcrossed with different inbred-lines for several successive generations to investigated the morphology response of flower to nuclear background.The result showed that：Ogu CMS take up the most part in the materials investigatied.Fourteen materials of 15 belong to this class.Only No.78 belong to Nap CMS.The response of Nap CMS to different nuclear background showed three types.The first type was similar with original material or show negative response;The second type was positive response;The third type was complicated,varying with floret parts.The Ogu CMS materials either showed a weak positive response to nuclear background or keep unchanged comparing to original material.Morphology response of different source of Ogu CMS to the same nuclear background was varied with unclear background.The inbred-line K1,N4 have similar impact on different cytoplasmic male sterile,but 37,F1 and G7 have different effect on different sources of cytoplasmic male sterile in flower morphology.