水通道蛋白5与低氧反应通路因子在鼻息肉组织中的表达及其相关性研究

Expression and the correlation of AQP5、HIF-1α and VEGF in human nasal polyps

目的:检测水通道蛋白5(AQP5)、缺氧诱导因子1α(HIF-1α)和血管内皮生长因子(VEGF)在鼻息肉组织中的表达情况,并探讨3种因子之间表达的相关性及其在鼻息肉发病机制中的作用。方法:取18例患者鼻息肉组织(鼻息肉组)及10例单纯行鼻中隔偏曲矫正术中切除的下鼻甲组织(对照组),分别运用RT-PCR和Western blot检测AQP5、HIF-1α、VEGF mRNA以及蛋白的表达情况;采用t检验和直线相关分析进行统计学分析。结果:①RT-PCR结果显示,AQP5 mRNA在鼻息肉组中的表达明显低于对照组,差异有统计学意义(P<0.01),HIF-1α、VEGF mRNA在鼻息肉组和对照组中表达差异无统计学意义(P>0.05);②Western blot结果显示AQP5蛋白表达鼻息肉组与对照组无明显差异(P>0.05),HIF-1、VEGF蛋白表达水平在鼻息肉组中的表达显著高于对照组(P<0.05);③Western blot结果显示鼻息肉组中AQP5与HIF-1α之间蛋白的表达呈明显正相关(r=0.633,P<0.01),AQP5与VEGF蛋白质表达亦呈明显正相关(r=0.611,P<0.01)。结论:AQP5与HIF-1α、VEGF等低氧反应通路因子共同参与鼻息肉组织水肿的形成。前者通过组织分布异常引起水转运障碍进而导致组织水肿形成,后者主要通过诱发血管增殖、增加血管通透性进而导致组织水肿产生;两者是相对独立的系统,推测其可能通过某种间接途径发生联系。

Objective：To detect the expression of AQP5,HIF-1α and VEGF in human nasal polyps,and to observe the relationship of AQP5 with HIF-1α and VEGF,and to investigate their role in the pathogenesis of nasal polyps.Method：Using the techniques of Real-time Quantitative Polymerase chain Reaction and Western blot,the expressions of AQP5,HIF-1α,VEGF mRNA and protein were examined.The specimens were obtained from patients underwent endoscopic surgery at the same time,including eighteen nasal polyps and ten inferior turbinate tissues.Result：①The result of RT-PCR showed that the expressions of the AQP5 mRNA were lower in nasal polyps than those in inferior turbinate tissue（P〈0.01）;there was no significant difference between the expression of HIF-1α mRNA or VEGF mRNA in nasal polyps and inferior turbinate tissues（P〈0.05）;②According to the Western blot,there was no significant difference between the expression of AQP5 in nasal polyps and inferior turbinate tissues（P〉0.05）;the expression of HIF-1α or VEGF were higher in nasal polyps than those in inferior turbinate tissues（P〉0.05）;③According to the Western blot,there was a positive correlation between the expression of AQP5 and that of HIF-1α（r=0.633,P〈0.05）,and the correlation was also existed between AQP5 and that of VEGF（r=0.611,P 0.05）.Conclusion：AQP5,HIF-1α,VEGF are all involved in the edema formation of nasal polyps.The changes of AQP5＇s distribution can cause accumulation of water in nasal polyps,HIF-1α,VEGF can induce new blood vessels and increase vasopermeability in nasal polyps;they have separate regulatory mechanism in edema formation,but might have some relationships in some ways.