丁酸钠增强5-aza-dC对人胃癌MKN-28细胞生长的抑制作用

Sodium butyrate enhances the inhibitory effect of 5-aza-2'-deoxycytidine on the growth of human gastric cancer MKN-28 cell lines

目的探讨丁酸钠(NaB)和5-氮-2′-脱氧胞苷(5-aza-dC)对胃癌细胞MKN-28细胞生长抑制的协同作用。方法 MKN-28细胞分为5-aza-dC组(A组)、NaB组(B组)、5-aza-dC+NaB组(C组)和对照组(D组)。四甲基偶氮唑盐比色(MTT)法观察人胃癌MKN-28细胞生长,流式细胞术检测细胞周期及细胞凋亡。结果 C组对MKN-28细胞的生长抑制作用明显强于A、B组(P<0.05);A组对细胞周期无影响,而B组及C组均可使细胞周期阻滞于G0/G1期(P<0.05);C组诱导的细胞凋亡率明显高于A、B组[(33.7±3.1)%vs.(8.3±1.3)%、(20.8±2.4)%](P<0.05)。结论 NaB可增强5-aza-dC对人胃癌MKN-28细胞的生长抑制作用,此协同作用可能与阻滞细胞周期及诱导细胞凋亡有关。

Objective To examine the synergic inhibitory effect of sodium butyrate（NaB） and demethylating agent 5-aza-2＇-deoxycytidine（5-aza-dC） on the cell growth of human gastric cancer cell line MKN-28.Methods The MKN-28 cells were divided into 4 groups of A（treated with NaB）,B（treated with 5-aza-dC）,C（treated with NaB plus 5-aza-dC） and D（without treatment as the control）.MTT assay was used to observe the cell cycle of cell growth and flow cytometry was used for analyzing the cell apoptosis.Results Compared with 5-aza-2＇-deoxycytidine or sodium butyrate alone,the inhibitory effect on the cell growth was higher in group C than that in groups of A and B（P〈0.05）.No significant change in cell cycle was seen in group A.The sell ratio of G0/G1 phase was significantly increased in groups of B and C（P〈0.05）.The cell apoptosis rate was higher in group C than that in groups of A and B[（33.7±3.1）% vs.（8.3±1.3）% and （20.8±2.4）%]（P〈0.05）.Conclusion Sodium butyrate enhances the inhibitory effect of 5-aza-2＇-deoxycytidine on the growth of human gastric cancer MKN-28 cell lines,which may be related to inducing apoptosis and arresting cell cycles.