唐山地区A组轮状病毒VP7基因差异分析

Analysis on differentiation of group A rotavirus VP7 gene in Tangshan district

目的调查唐山地区A组轮状病毒感染现况。方法 RT-PCR鉴定唐山地区A组轮状病毒流行株型别,提取病毒总RNA,扩增目的基因片段VP7,分离纯化和回收,连接pMD18-T载体,转化质粒进行抽提与鉴定,同时进行基因测序。结果与标准病毒株进行基因差异分析。结果成功连接VP7基因与pMD18-T载体。基因测序分析结果 :唐山地区A组轮状病毒VP7基因与标准病毒株VP7基因序列符合率(同源性)为98.5%,其关键基因没有发生变异。结论唐山地区A组轮状病毒VP7基因的关键基因没有发生变异。

Objective To investigate the infection of group A rotavirus in Tangshan district.Methods The samples from people in Tangshan district were collected for identifying VP7 genotypes and extracting the total RNA of group A rotavirus RNA by RT-PCR,which was amplified by RT-PCR and transducted into pMD18-T after isolated and purified.The results of sequence analysis were compared with standard group A rotavirus.Results The VP7 gene was transformed successfully into pMD18-T.The sequence analysis confermed that the accordance rate of VP7 gene with standard group A rotavirus was 98.5% and no differentiation of key gene occurred.Conclusion The VP7 gene in Tangshan district has no differentiation of key gene.