辽宁省鼠类感染肾综合征出血热的病原学及分子生物学检测比较

Nosetiology and molecular biology analysis of rats infected with HFRS in Liaoning Province

目的了解辽宁省鼠间带毒、鼠密度及鼠种构成与汉坦病毒的分子特征。方法采用夹夜法对鼠密度、鼠种构成及鼠带毒率进行监测,用间接免疫荧光方法检测HFRS抗原,用Vero-E6细胞对病毒进行传代分离,RT-PCR方法对阳性毒株进行基因分型及测序分析。结果鼠密度在春秋季各出现一个高峰,村内鼠密度高于村外鼠密度,褐家鼠为优势鼠种。带毒率无显著性差异,各鼠种间带毒率无显著性差异。5株病毒,有2个遗传分支,4株为HV的SEO型,1株为HTN型,4株SEO型HV基本位于系统发生树的同一进化枝上;1株HTN型HV,与汉滩病毒标准株76-118接近。结论辽宁省每年存在春秋季两个鼠密度高峰,带毒率同季节及鼠种无关。辽宁省是以SEO型HV为主的SEO和HTN混合型疫区,且SEO型汉坦病毒变异较小。

To understand rat density,species constitution,positive rate of hemorrhagic fever with renal syndrome（HFRS）and molecular characteristic of Hantaviruses（HV）in Liaoning Province,a surveillance was conducted with night trapping method.The HFRS antigen was detected by IFAT and the lung cell culture was conducted with Vero-E6 cells.Then the amplified products were sequenced by RT-PCR.Results showed that there were two peak values on rat density within twelve months.The rat density was higher in village than in open country,and the sewer rats were dominant.There was no significant difference in positive rate of HFRS for four seasons or for different species of rats.The five HVs were identified into two genetic branches-four for SEO and one for HTN.The four SEO viruses were nearly in the same evolutionary branch and the only HTN virus was close to HTN76-118.In conclusion,there were two rat density summits per year in Liaoning Province and there was no relationship between the positive rate of HFRS and the species constitution or season.Liaoning province was a complex area with both SEO and HTN virus.The SEO virus was dominated and the genovariation was relatively weak.