DNA芯片鉴定分枝杆菌的研究

Rapid identification of Mycobacterium species by DNA chip

目的评价DNA芯片技术鉴定分枝杆菌菌种的应用价值,为临床实际应用提供科学依据。方法收集以BACTECMGIT960从结核病患者分离到的分枝杆菌阳性培养物,以传统分枝杆菌菌种鉴定方法为对照,应用DNA芯片技术进行菌种鉴定。结果两种方法鉴定结果一致和基本一致共112株,吻合率为83.6%(112/134),包括胞内分枝杆菌50株,龟分枝杆菌14株,结核分枝杆菌14株,戈登分枝杆菌9株,鸟分枝杆菌7株,偶然分枝杆菌7株,堪、瘰、胃和猿分枝杆菌6株,土分枝杆菌和海分枝杆菌各1株;未分类NTM3株。应用DNA芯片未能分类的17株分枝杆菌中,传统方法分别鉴定为戈登分枝杆菌5株、胞内分枝杆菌3株、龟分枝杆菌2株、蟾分枝杆菌、耻垢分枝杆菌和浅黄分枝杆菌各1株,未分类NTM4株。结论用DNA芯片检测技术,可以简便、快速、灵敏、特异地将大多数分枝杆菌鉴定到种,但有待进一步完善。

The aim of the study was to evaluate the clinical value of identification of Mycobacterium species by DNA chip,and provide scientific basis for clinical application.Clinical samples were cultured by BACTEC MGIT 960,and the species of 134 Mycobacterium isolates were identified by traditional identification methods and DNA chip,respectively.The consistent rate of Mycobacterium isolates identified by these two methods was 83.6%（112/134）and including 50 M.intracellulare,14 M.chelonae,14 M.tuberculosis,9 M.gordonae,7 M.avium,7 M.fortuitum 1 M.terrae,1 M.marinum,and 3 unidentified isolates.Of 17 Mycobacterium isolates could not be identified by DNA chips,in which 5 M.gordonae,3 M.intracellulare,2 M.chelonae,1 M.xenopi,1 M.smegmatis and 1 M.gilvum were identified by traditional identification methods.While 4 isolates could not be identified by both of these methods.It is concluded that DNA chip might be a simple,rapid,sensitive and specific method for identification of majority Mycobacterium species.