转化生长因子β1对体外培养尿道细胞的生物学作用研究

Biologic effect of transforming growth factor-β1 on urethra cells cultured in vitro

目的 观察转化生长因子β1（TGF-β1）对尿道黏膜上皮细胞及成纤维细胞的生长调节作用及在诱导靶细胞结缔组织生长因子（CTGF）的表达中的作用.方法 体外培养尿道黏膜上皮细胞及成纤维细胞并鉴定.取第4代细胞分别设立对照组（以不含TGF-β1的细胞培养液培养）和实验组（分别以TGF-β11、2、4、8 μg/L的细胞培养液培养）,24 h后分别以MTT比色试验和细胞计数法检测细胞活力,RT-PCR检测细胞内CTGF mRNA的表达.结果 与对照组相比较,实验组尿道黏膜上皮细胞数量和吸光度值随TGF-β1浓度升高而降低（P＜0.05）,成纤维细胞数量和吸光度值随TGF-βl浓度升高而升高（P＜0.05）;CTGF mRNA在实验组上皮细胞和成纤维细胞中均有表达,且表达水平随TGF-βl浓度升高而增加（P＜0.05）.结论 TGF-β1可抑制尿道黏膜上皮细胞生长,促进成纤维细胞生长,并能诱导尿道黏膜上皮细胞及成纤维细胞表达CTGF mRNA.

Objective To investigate the effects of transforming growth factor-βl （TGF-β1） on growth controling and the expression of connective tissue growth factor mRNA （CTGF mRNA） in urethra epithelium cells and fibroblasts cultured in vitro. Methods Urethra epithelial cells and fibroblasts were culturedin vitro and identified. The fourth generation cells were divided into control group（ cultured by cell medium without TGF-βl ） and experimental groups（ cultured by cell medium containing TGF-β1 1,2, 4 and 8 μg/L）, the vital force of cells were examined by MTT and cell counting, the expression of CTGF mRNA were examined by RT-PCR after 24 hours. Results The optical density and cell count decreased in experimental groups of urethra epithelium cells and increased in experimental groups of fibroblasts with the concentration of TGF-β1 being heightened compared with the control group（P〈0.05 ）. The expression of CTGF mRNA increased with the heightening concentration of TGF-β1 in all experimental groups of urethra epithelium cells and fibroblasts by RT-PCR （P 〈 0. 05 ）. Conclusions TGF-β1 can inhibit the growth of urethra epithelium cells and promote the growth of fibroblastsin vitro, it can induce the expression of CTGF mRNA in two cells above-mentioned.