核素标记自体骨髓单个核细胞监测心脏干细胞移植后存活情况

Feasibility of in vivo isotope labeled autologous mononuclear bone marrow cells monitoring post cardiac transplantation in a swine model of myocardial infarction

目的^18F-氟代脱氧葡萄糖（^18F-FDG）标记自体骨髓单个核细胞,进行无创性的显像,并评价移植后干细胞在体内的分布和存活情况.方法 中华小型猪8只麻醉后结扎冠状动脉前降支建立急性心肌梗死模型,经髂骨抽取骨髓20 ml,分离骨髓单个核细胞后用放射性核素^18F-FDG体外标记,以心肌内直接注射的方式注入梗死区,进行即刻、1 h、2 h和3 h全身平面显像和心肌断层显像.体外锥虫蓝染色检测放射性核素标记后3 h的细胞活力.结果 ^18F-FDG体外标记率为（67±14）%,锥虫蓝染色活细胞率为（95±3）%.标记后的骨髓单个核细胞注入心脏后,即刻、1 h、2 h和3 h在梗死区内的储留比例分别为（83±6）%、（49±8）%、（32±6）%和（24±5）%.结论 利用^18F-FDG标记骨髓单个核细胞,可以有效监测干细胞心脏移植后急性期在体内的分布和在心脏内的储留数量.

Objective To observe in vivo stem cell distribution and viability after transplantation by noninvasive imaging of ^18F-fluorodeoxyglucose（ ^18F-FDG）labeled autologous mononuclear bone marrow cells.Methods Myocardial infarction was established in 8 swine by ligating left anterior descending coronary artery after anesthesia. Bone marrow （20 ml） was drawn through ileum. After isolation, mononuclear bone marrow cells were labeled by radionuclide ^18F-FDG and intramyocardially injected into infarction region. Whole body planar scan and myocardial tomography scan were performed immediately, 1 h, 2 h, and 3 h post stem cell injection. Viability and stability of radionuclide labeled stem cells were determined at 3 h post labeling in vitro. Results The labeling efficiency was （67 ± 14）%. Mean dose of radioactive in marrow cells was （32 ±7）MBq. Trypan blue staining showed in vitro viability was （95 ±3）% at 3 h post labeling.After intramyocardial injection, labeled mononuclear bone marrow cell retention rate in infarction region was （83 ± 6） %, （49 ± 8 ） %, （ 32 ± 6） % and （24 ± 5 ） % immediately, 1 h, 2 h, and 3 h post injection, respectively. Conclusions Distribution and viability of stem cell after cardiac transplantation could be effective monitored by ^18F-FDG labeled autologous mononuclear bone marrow cell technique in acute stage in this model.