β2AR、β-arrestin2、NF-κBp65在溃疡性结肠炎大鼠中的表达及乌梅丸的干预作用

Wumeiwan treatment alters the expression of β2AR, β-arrestin 2 and NF-κB p65 in rat ulcerative colitis

目的:检测溃疡性结肠炎大鼠在药物治疗前后β2AR、β-arrestin2、NF-κBp65的表达水平.方法:SD大鼠随机分为4组:空白组、模型组、美沙拉秦西药组和乌梅丸中药组.模型成功建立后空白组和模型组以生理盐水3mL灌胃,美沙拉秦组用50g/L的美沙拉秦混悬液50mg/100g灌胃,乌梅丸组给予0.515g/mL的乌梅丸液3mL灌胃,各组均连续给药15d后,取脾脏和结肠组织分别用Westernblot法和免疫组织化学法检测β2AR、β-arrestin2、NF-κBp65的表达.结果:在正常组织中β2AR、β-arrestin2的表达较多(15.28%±1.71%,15.28%±1.58%),模型组二者的表达明显下降(12.54%±1.28%,12.67%±1.42%),经治疗后,乌梅丸组和美沙拉秦组中二者的表达显著增加(16.27%±1.40%,16.18%±1.12%;17.05%±1.48%,16.77%±1.40%),免疫组织化学法检测显示表达率有统计学意义.而NF-κBp65在正常组织中表达较少,模型组NF-κBp65的表达明显增加(17.79%±1.24%),经药物治疗后其在乌梅丸组和美沙拉秦组中表达明显下降(16.61%±1.42%,15.39%±1.21%),免疫组织化学法检测显示表达率有统计学意义.结论:对于溃疡性结肠炎的治疗,乌梅丸和美沙拉秦均有明显疗效.

AIM： To investigate the effects of Wumeiwan treatment on the expression of β2-adrenergic receptor （β2AR）, β-arrestin 2, NF-κB p65 in ulcerative colitis in rats. METHODS： Sprague-Dawley rats were randomly divided into four groups： control group,model group, mesalazine group and Wumeiwan group. Ulcerative colitis was induced in rats with 2, 4, 6-trinitrobenzenesulfonic acid. The control group and model group were administered intragastrically with 3 mL of normal saline, while the mesalazine group and Wumeiwan group were intragastrically given mesalazine at a dose of 50 mg/100 g body weight and 3 mL of Wumeiwan （0.515 g/mL）, respectively. All rats were treated for 15 d. Spleen and colon tissue samples were taken to detect the expression of β2AR, β-arrestin 2 and NF-κB p65 by Western blot and immunohistochemistry. RESULTS： The expression levels of β2AR and β-arrestin 2 were significantly lower in the model group than in the control group （12.54% ± 1.28% vs 15.28% ± 1.71%, 12.67% ± 1.42% vs 15.28% ± 1.58%）. Treatment with either Wumeiwan or mesalazine significantly increased the expression of β2AR and β-arrestin 2 （16.27% ± 1.40%, 16.18% ± 1.12%; 17.05% ± 1.48%, 16.77% ± 1.40%）, and there were no signifi cant differences in the expression levels of β2AR and β-arrestin 2 between the Wumeiwan and mesalazine groups. The expression level of NF-κB p65 was signifi cantly higher in the model group than in the control group （17.79% ± 1.24% vs 13.82% ± 1.13%）. Treatment with either Wumeiwan or mesalazine signifi cantly decreased the expression of NF-κB p65 （16.61% ± 1.42%, 15.39% ± 1.21%）, and there was no significant difference in the expression level of NF-κB p65 between the Wumeiwan and mesalazine groups. CONCLUSION： Both Wumeiwan and mesalazine have signif icant effi cacy in the treatment of ulcerative colitis in rats.