十二指肠灌注生大黄对SD大鼠胰腺外分泌的影响

Effect of intraduodenal infusion of rhubarb on pancreatic exocrine secretion in Sprague-Dawley rats

目的:观察十二指肠灌注生大黄对SD大鼠胰腺外分泌的影响.方法:取体质量250-300gSD大鼠24只,随机分为2组(n=12).麻醉后在大鼠幽门下5mm和十二指肠、空肠交界处分别置直径2mm的塑料管并固定;从十二指肠乳头Oddi括约肌处留置直径0.7mm的塑料留置管.稳定30min后,每隔15min收集1次胆胰混合液,测定体积后留取100μL胆胰混合液用双蒸水稀释以测定蛋白质含量,剩余的胆胰混合液在下一收集时间内灌入十二指肠.收集5管基础状态胆胰混合液后实验组以3mL/h的速度向十二指肠内灌注生大黄2h,并收集8管胆胰混合液进行体积和蛋白质测定.对照组不进行十二指肠灌注.结果:十二指肠灌注生大黄后胆胰混合液体积较基础状态增加30.95%,与对照组比较,差别有统计学意义(P<0.01);蛋白质分泌量较基础状态增加5.02%,但与对照组比较,其差别无统计学意义(P>0.05).结论:对大鼠进行十二指肠灌注生大黄后单位时间内胆胰混合液分泌的体积增加,但蛋白质分泌量无明显增加.

AIM： To observe the effect of intraduodenal infusion of rhubarb on pancreatic exocrine secretion in Sprague-Dawley （SD） rats. METHODS： Twenty-four SD rats were random- ly and equally divided into two groups： experi- mental group and control group. After anestheti- zation, a cannula was inserted into the sphincter of oddi. A segment of small intestine, including the whole duodenum, was isolated between the first intestinal cannula positioned 5 mm from the pylorus and the second intestinal cannula placed at the boundary between the duodenum and jejunum. After a 30-min stabilization period, the combined bile-pancreatic secretions were collected every 15 min for 5 times. The pancre- atic fluid volume was measured, and an aliquot was taken and diluted with distilled water for protein determination. The remaining undiluted bile-pancreatic juice was pumped back into theduodenum during the next collection period. Then intestinal perfusion of 20% intralipid was performed in the experimental group at a speed of 3 mL/h for 2 h, and the combined bile-pancreatic secretions were collected and the pancreatic fluid flow and protein secretion were measured. The control group underwent no intestinal infusion. RESULTS： After intraduodenal infusion of rhu- barb, the volume of combined bile-pancreatic secretions increased by 30.95% and the protein output increased by 5.02% over the baseline lev- els. In the experimental group, there was a sig- nif icant increase in the volume of combined bile- pancreatic secretions compared with the normal control group （P 0.01）. No signif icant increase was noted in protein output between the two groups （P 0.05）. CONCLUSION： Intraduodenal infusion of rhu- barb can increase the volume of combined bile- pancreatic secretions in SD rats but had no sig- nifi cant impact on protein output.