摘要
目的和方法：对大鼠肺微血管内皮细胞（ＰＭＶＥＣ）培养、鉴定。观察了低浓度Ｈ２Ｏ２（０～１００μＭ）对体外培养的ＰＭＶＥＣ的迟发性损伤效应。结果：ＭＴＴ法发现Ｈ２Ｏ２对ＰＭＶＥＣ的增殖代谢活性具有剂量依赖性抑制作用，而且当Ｈ２Ｏ２去除后，此作用仍可进一步发展。形态观察、流式细胞仪ＤＮＡ测定、３’末端标记ＤＮＡ降解片断原位检测（ＴＵＮＥＬ）均表明，细胞凋亡是Ｈ２Ｏ２对ＰＭＶＥＣ迟发性损伤效应的一种重要形式。
AIM and METHOD:Pulmonary microvascular endothelial cells (PMVEC) were cultured and characterized.Delayed injury effects of low concentration of H 2O 2(0～100 μmol/L)on the PMVECs were observed.RESULTS:It was found with MTT method that H 2O 2 inhibited the proliferative and metabolic activity of PMVEC in vitro in a dose-dependent way,and after the removal of H 2O 2,this effect could develop further.Morphological observation,flow cytometry and DNA fragment in situ detection (TUNEL) revealed that apoptosis is an important form of delayed injury effect of H 2O 2 on the endothelial cells.CONCLUSION:Low concentration of H 2O 2 could induce a delayed effect on PMVEC: apoptosis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第1期28-32,共5页
Chinese Journal of Pathophysiology
基金
美国中华医学基金
