SHR血浆NPY、主动脉平滑肌细胞NPY受体的变化及药物的干预作用

Studies on the plasma NPY levels,the distribution of NPY receptor on aortic vascular smooth muscle cells in SHR

目的：观察自发性高血压大鼠（ＳＨＲ）血浆神经肽Ｙ（ＮＰＹ）、血管平滑肌细胞（ＶＳＭＣ）ＮＰＹ受体的变化及美托洛尔、培哚普利干预的影响。方法：用放射免疫分析法和受体放射配体结合分析法测定血浆ＮＰＹ及ＶＳＭＣ上ＮＰＹ受体密度的变化。结果：ＳＨＲ血浆ＮＰＹ含量明显高于ＷＫＹ，血压的高低与血浆ＮＰＹ的量呈明显的正相关。培哚普利降压的同时亦降低ＳＨＲ血浆ＮＰＹ的量，而美托洛尔对ＮＰＹ量则无影响；培哚普利引起ＮＰＹ受体密度上调，而美托洛尔则使ＮＰＹ受体下调。结论：ＮＰＹ在高血压的发生、发展中可能起到重要的作用；在培哚普利的降压作用中可能有血浆ＮＰＹ含量下降这一因素参与，美托洛尔致ＮＰＹ受体密度下调可能在预防主动脉管壁结构重建中发挥着一定的作用。

AIM:To investigate the changes of plasma neuropeptide Y (NPY) levels, the NPY receptors on aortic vascular smooth muscle cells and the effects of perindopril,metoprolol upon spontaneuosly hypertensive rats(SHR).METHODS:NPY plasma levels and receptors were detected by radioimmunoassay and radioligand binding assay.RESULTS:Plasma NPY levels were significantly higher in SHR than that in Wistar-Kyoto rats(WKY);There was a close relationship between the height of blood pressure and plasma NPY levels.Perindopril could markedly inhibit release of NPY through the prejunctional facilitary angiotensin Ⅱ receptors,while the metoprolol could not.Perindopril induced upregulation of NPY receptor density;metoprolol induced downregulation of NPY receptor density.CONCLUSIONS:NPY might be involved in the pathogenesis of hypertension,the decrease of NPY levels might be involved in the antihypertensive mechanism of perindopril.Metoprolol induced downregulation of NPY receptor density,which might play an important role in the prevention of hypertrophy of vascular smooth muscle cells.