L-精氨酸对缺氧性肺动脉高压大鼠内皮素释放的影响

Effect of L-arginine on release of endothelin in hypoxic pulmonary hypertensive rats

目的：探讨Ｌ－精氨酸（Ｌ－Ａｒｇ）对缺氧性肺动脉高压（ＨＰＨ）大鼠血浆内皮素－１（ＥＴ－１）释放的影响。方法：将Ｗｉｓｔａｒ大鼠４０只分为：对照组，缺氧组，缺氧＋Ｎω－硝基－Ｌ－精氨酸甲脂（Ｌ－ＮＡＭＥ）组和缺氧Ｌ－Ａｒｇ组。结果：缺氧组的肺动脉平均压（ｍＰＡＰ）显著高于对照组（Ｐ＜０．０５），缺氧组＋Ｌ－Ａｒｇ组的ｍＰＡＰ显著低于缺氧组（Ｐ＜０．０５）及缺氧＋Ｌ－ＮＡＭＥ组（Ｐ＜００１），缺氧组的ＲＶ／（ＬＶ＋Ｓ）比值显著高于对照组（Ｐ＜００１），缺氧＋Ｌ－ＮＡＭＥ组的ＲＶ／（ＬＶ＋Ｓ）比值显著高于缺氧组（Ｐ＜００５）及缺氧＋Ｌ－Ａｒｇ组（Ｐ＜００１），缺氧组和缺氧＋Ｌ－ＮＡＭＥ组ＥＴ－１含量明显高于对照组和缺氧＋Ｌ－Ａｒｇ组（Ｐ均＜００１），ｍＰＡＰ与血浆ＥＴ－１含量呈明显正相关（ｒ＝０６８２，Ｐ＜００５）。结论：Ｌ－Ａｒｇ在ＨＰＨ大鼠中，通过影响ＥＴ－１的释放调节ＨＰＨ。

AIM:To investigate effect of exogenous L-arginine(L-Arg) on release of endothelin in hypoxic pulmonary hypertension(HPH)rats.METHODS:Forty rats were randomly divided into four groups:control group,hypoxic group,hypoxia+L-NAME group and hypoxia+L-Arg group.Pulmonary artery mean pressure(mPAP),dry weights of right ventricle(RV),left ventricle+septum(LV+S)were measured and RV/(LV+S)ratio was calculated.Plasma endothelin-1 (ET-1) concentration was detected by using RIA.RESULTS:mPAP was significantly higher in rats of hypoxic group than that of control group( P <0 05) and it was much lower in rats of hypoxia+L-Arg group than that of either hypoxic group ( P <0 05) or hypoxia+L-NAME group( P <0 05).Weight of RV/(LV+S)ratio was increased in rats of hypoxic group compared with that of control group( P <0 01).While,it was increased in rats of hypoxia+L-NAME group compared with that of either hypoxic group( P <0 05)or hypoxia+L-Arg group( P <0 01).Plasma ET-1 level in rats of either hypoxic group or hypoxia+L-NAME group was higher than that of either control group or hypoxia+L-Arg group( P <0 01).mPAP positively correlated with plasma ET level( r =0 682, P <0 05) CONCLUSION:L-Arg played a modulatory role on the development of HPH through impacting the ET release from hypoxic rats.