慢性缺氧和二氧化碳潴留大鼠脑阴离子转运蛋白的研究

Chronic hypoxia and hypercapnia on the expression of anion exchanger mRNA in rat brains

目的：观测慢性缺氧和二氧化碳潴留大鼠脑桥组织中脑阴离子转运蛋白（ＡＥ３）ｍＲＮＡ的变化。方法：采用密闭舱内灌注混合气体的方法，分别复制慢性缺氧，慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒大鼠模型，以地高辛标记的（ＡＥ３）ｃＤＮＡ探针，应用点杂交技术，对各实验组及对照组大鼠脑桥组织中ＡＥ３ｍＲＮＡ的相对含量进行测定。结果：（１）慢性缺氧组ＡＥ３ｍＲＮＡ的相对含量明显地低于对照组（Ｐ＜００５），慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒组的ＡＥ３ｍＲＮＡ降低更为明显；（２）慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒二组之间无显著差异（Ｐ＞００５），但都明显低于慢性缺氧组（Ｐ＜００１）。结论：提示ＡＥ３在呼吸衰竭及其导致的肺性脑病的发生中可能具有一定作用。

AIM:To study the expression of anion exchanger (AE 3) of brain in rats under chronic hypoxia and hypercapnia.METHODS:With a sensitive digoxigenin labelled AE 3 cDNA probe,dot blot was used to evaluated changes of AE 3 mRNA in rats models of chronic hypoxia,chronic hypoxia with hypercapnia and chronic acidosis established by pouring mixed gases into the chamber respectively as compared with control.RESULTS:It was found that the relative contents of AE 3 mRNA in rat brains in experimental group were significantly lower than that in control group( P <0 05),and the relative contents of AE 3 mRNA in rat brains of hypoxia with hypercapnia and chronic acidosis were also significantly lower than that of chronic hypoxia( P <0 01),but there was no significant difference between chronic hypoxia with hypercapnia group and chronic acidosis group( P >0 05).CONCLUSION:AE 3 may play an important role in the respiratory failure and pulmonary encephalophathy.