摘要
目的和方法:用封闭群新西兰家兔56只,分为7组,注射不同pH(40,60,74,100)的甘氨酸和内毒素混合液,观察注射后体温变化,由此反映出不同pH对甘氨酸拮抗内毒素致热作用的影响。结果:甘氨酸的拮抗效果在碱性溶液中减弱、酸性溶液中增强,这种变化与pH对甘氨酸NH+3端解离程度的影响是一致的。进一步用氯甲酸苄脂封闭甘氨酸的氨基端,通过鲎试验方法观察到,封闭氨基端后甘氨酸对内毒素的拮抗作用消失。结论:甘氨酸拮抗内毒素的机制可能是通过其带正电荷的NH+3端实现的,NH+3与内毒素脂质A上的PO4结合,干扰了内毒素的正常结构。
AIM and METHODS: Fifty-six healthy New Zealand rabbits were randomly divided into 7 groups. The body temperature changes of rabbits were observed after intravenous injection of mixed solution of glycine-endotoxinwith different pH values (4 0、6 0、7 4、10 0). RESULTS:The effect of glycine on endotoxin was decreased in basic solution and increased in acidic solution, which was in accordance with the effect of pH on the dissociation of glycine amino terminal (NH + 3). Furthermore, using TAL test method, it was found that the anti-endotoxin effect of glycine disappeared after sealing of its amino terminal.CONCLUSION:The mechanism of antagonism of glycine against endotoxin may be attributed to its positive amino terminal which may combine with the PO 4 group of lipid A of endotoxin and interfere with structural integrity of endotoxin.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第2期110-112,共3页
Chinese Journal of Pathophysiology
基金
国家自然科学基金
关键词
甘氨酸
内毒素
PH值
拮抗作用
Glycine
Endotoxins. Hydrogen, ion concentration