豚鼠延髓c-fos基因表达在钩端螺旋体OmpL_(39)免疫应答期的变化

Changes of the expression of the c- fos of the bulb of quinea pigs in the immune response of OmpL 39 from leptospira

目的和方法：观察钩体外膜疏水蛋白ＯｍｐＬ３９的免疫保护作用和对豚鼠中枢ｃ－ｆｏｓ基因表达的影响，分别用ＯｍｐＬ３９与钩体死菌苗（ＷＬＣＶ）、生理盐水（ＮＳ）对照免疫豚鼠，用强毒０１７株钩体活菌攻击，处死动物后用新鲜延髓切片观察对比。结果：ＯｍｐＬ３９在豚鼠体内能产生高效价的阳性抗体，免疫保护率为１００％。ＯｍｐＬ３９免疫组豚鼠中枢ｃ－ｆｏｓ基因表达面积比为０．００８６％±０．０００３％，颗粒计数为４６３．０±１．３４个，较ＷＬＣＶ和ＮＳ组显著减少（Ｐ＜０．０５）。结论：ＯｍｐＬ３９有较强的免疫保护作用，对豚鼠中枢ｃ－ｆｏｓ基因的表达有抑制作用。

AIM and METHODS To study the immunoprotection and the related mechanism of OmpL 39 and the effect of idiosyncrasy antigen on the c- fos expression in the bulb of the guinea pigs. We immunized the guinea pigs separately with OmpL 39 , whole Leptospira cell vaccine(WLCV) and normal saline (NS) and then attacked them with virulent strain 017.RESULTS:OmpL 39 could produce high-valence positive antibody (MAT 1:>3200). The immunoprotective rate was 100%. The c- fos gene expression granule area rate was 0.0086%±0.0003%, and the granule count was 4.63±1.34. The c- fos gene expression of the guinea pigs of OmpL 39 group was remarkably less than that of the control group (WLCV and NS) ( P <0.05).CONCLUSION:OmpL 39 could specifically inhibit the expressions the c- fos in the bulb of the guinea pigs. It was more effective in immunoprotection than WLCV.