摘要
目的:探讨血管运动产生机制及其与电压依赖性钙通道的关系。方法:健康雄性大耳白家兔20只,体重:20~25kg,以乌拉坦(ip)为基础麻醉,α-氯醛酮(iv)为维持麻醉,应用显微录像静画步进法观察家兔肠系膜局部施加去甲肾上腺素(NA)08μg后,诱发出微动脉周期性、节律性的血管运动及静脉注入尼卡地平对其产生的影响。结果:微动脉血管运动频率:(708±064)counts/min,振幅:(551±042)μm。血管运动的振幅在同一支微动脉上分布不均,呈节段性。尼卡地平025g·L-1·kg-1和05g·L-1·kg-1均扩张微动脉内径、降低血管运动频率及振幅。
AIM:To further discuss the mechanism of vasomotion and probable relationship between voltage-dependent calcium channel and vasomotion.METHODS:20 male albino rabbits weighting 2.0~2 5 kg each were used for the experiment.They were anesthetized with 25% urethan (0.75g/ kg) and α-chloralose (30 mg/ kg).TV Frame-to-Frame analysing method was used to observe the characteristics of arteriolar vasomotion in rabbit mesentery caused by topical application of NA 0 8 μg,as well as the effects of intravenous injection of nicardipine on vasomotion frequency and amplitude.RESULTS:Topical application of NA 0.8μg was conducted to the observation field,vasomotion occured with frequency (7 08±0 54)cpm,amplitude (5 51±0 42)μm;amplitude was not uniform on the same arteriole.Nicardipine at the dosage of 0.25g·L -1 ·kg -1 and 0.50g·L -1 ·kg -1 dilated the diameter and inhibited vasomotion with reduced frequency and amplitude.CONCLUSION:The mechanism underlying vasomotion is related to voltage-dependent calcium channels.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第2期119-121,共3页
Chinese Journal of Pathophysiology
基金
国家自然科学基金