一氧化氮对离体心脏功能的影响

Effects of nitric oxide on myocardial function in isolated working heart preparation

目的：初步探讨一氧化氮对心脏功能的作用及其可能机制。方法：本实验利用Ｌａｎｇｅｎｄｏｒｆ离体工作心脏灌流装置及技术，观察了ＮＯ供体ＳＩＮ－１、硝普钠灌流心脏后心功能及心肌某些生化指标的改变情况。结果：心脏功能多项指标明显下降，以ＬＶＳＰ，±ｄｐ／ｄｔｍａｘ为甚。伴之心肌ｃＧＭＰ含量的升高及心肌细胞膜Ｎａ＋－Ｋ＋－ＡＴＰ酶活性的下降。结论：一氧化氮通过调控心肌ｃＧＭＰ的合成及肌膜Ｎａ＋－Ｋ＋－ＡＴＰ酶活性而影响心肌功能。

AIM:To investigate the role of nitric oxide(NO) in cardiac function. METHOD:Changes of myocardial function and cyclic GMP,membrane Na +-K +-ATPase activity in myocardium were observed in isolated working heart preparation perfused with NO donor SIN-1, or sodium nitroprusside RESULTS:It was found that, parallel with marked decrease in cardiac function parameters (esp left ventricular systolic pressure, ±d p /d t max), an obvious elevation in cyclic GMP level was demonstrated with a declination of membrane Na +-K +-ATPase activity in myocardium. CONCLUSION:Nitric oxide might be involved in cardiac dysfunction by modulating cyclic GMP formation and Na +-K +-ATPase activity in myocardium.