摘要
以翅荚木的腋芽以及翅荚木幼苗嫩茎为外植体,进行组培快繁技术研究,结果表明:翅荚木适宜的初代培养基为MS+6-BA 1.0 mg.L-1+IBA 0.05 mg.L-1;继代培养基以MS+6-BA 0.5 mg.L-1+IBA0.1 mg.L-1+KT0.5 mg.L-1培养,效果好;生根培养则以MS+IBA0.05 mg.L-1+NAA0.05 mg.L-1+KT0.1 mg.L-1培养基的生根效果最好。
Taking seedling's axillary bud above stem and its the seed plants of terminal bud form Zenia insignis as explants,techniques of tissue culture were studied.The results show that the most suitable primary culture medium for Zenia insignis was MS+6-BA 1.0 mg·L-1+IBA 0.05 mg·L-1;propagation culture medium was MS+6-BA 0.5 mg·L-1+IBA 0.1 mg·L-1+KT 0.5 mg·L-1 better than others;the best root culture medium was MS+IBA 0.05 mg·L-1 +NAA 0.05 mg·L-1 +KT 0.1 mg·L-1.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2010年第6期60-63,共4页
Journal of Central South University of Forestry & Technology
基金
浙江省科技计划项目"珍稀速生原料林树种--翅荚木的引种及其繁育技术研究"(2005C32044)的部分研究内容
关键词
翅荚木
组织培养
快繁
Zenia insignis
tissue culture
rapid propagation