翅荚木组培快繁技术的研究

Study on tissue culture and rapid propagation in Zenia insignis

以翅荚木的腋芽以及翅荚木幼苗嫩茎为外植体,进行组培快繁技术研究,结果表明:翅荚木适宜的初代培养基为MS+6-BA 1.0 mg.L-1+IBA 0.05 mg.L-1;继代培养基以MS+6-BA 0.5 mg.L-1+IBA0.1 mg.L-1+KT0.5 mg.L-1培养,效果好;生根培养则以MS+IBA0.05 mg.L-1+NAA0.05 mg.L-1+KT0.1 mg.L-1培养基的生根效果最好。

Taking seedling＇s axillary bud above stem and its the seed plants of terminal bud form Zenia insignis as explants,techniques of tissue culture were studied.The results show that the most suitable primary culture medium for Zenia insignis was MS＋6-BA 1.0 mg·L-1＋IBA 0.05 mg·L-1;propagation culture medium was MS＋6-BA 0.5 mg·L-1＋IBA 0.1 mg·L-1＋KT 0.5 mg·L-1 better than others;the best root culture medium was MS＋IBA 0.05 mg·L-1 ＋NAA 0.05 mg·L-1 ＋KT 0.1 mg·L-1.